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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 6 — Mar. 15, 2010
  • pp: 6396–6407

Optical chromatography using a photonic crystal fiber with on-chip fluorescence excitation

P. C. Ashok, R. F. Marchington, P. Mthunzi, T. F. Krauss, and K. Dholakia  »View Author Affiliations

Optics Express, Vol. 18, Issue 6, pp. 6396-6407 (2010)

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We describe the realization of integrated optical chromatography, in conjunction with on-chip fluorescence excitation, in a monolithically fabricated poly-dimethylsiloxane (PDMS) microfluidic chip. The unique endlessly-single-mode guiding property of the Photonic Crystal Fiber (PCF) facilitates simultaneous on-chip delivery of beams to perform optical sorting in conjunction with fluorescence excitation. We use soft lithography to define the chip and insert the specially capped PCF into it through a predefined fiber channel that is intrinsically aligned with the sorting channel. We compare the performance of the system to a standard ray optics model and use the system to demonstrate both size-driven and refractive index-driven separations of colloids. Finally we demonstrate a new technique of enhanced optofluidic separation of biological particles, by sorting of human kidney embryonic cells (HEK-293), internally tagged with fluorescing microspheres through phagocytocis, from those without microspheres and the separation purity is monitored using fluorescence imaging.

© 2010 OSA

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.4520) Medical optics and biotechnology : Optical confinement and manipulation
(350.4855) Other areas of optics : Optical tweezers or optical manipulation

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: February 8, 2010
Revised Manuscript: March 4, 2010
Manuscript Accepted: March 4, 2010
Published: March 12, 2010

Virtual Issues
Vol. 5, Iss. 7 Virtual Journal for Biomedical Optics

P. C. Ashok, R. F. Marchington, P. Mthunzi, T. F. Krauss, and K. Dholakia, "Optical chromatography using a photonic crystal fiber with on-chip fluorescence excitation," Opt. Express 18, 6396-6407 (2010)

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