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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 8 — Apr. 12, 2010
  • pp: 7857–7871

Multiphoton excitation characteristics of cellular fluorophores of human skin in vivo

Hans Georg Breunig, Hauke Studier, and Karsten König  »View Author Affiliations


Optics Express, Vol. 18, Issue 8, pp. 7857-7871 (2010)
http://dx.doi.org/10.1364/OE.18.007857


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Abstract

In vivo multiphoton tomography with a wavelength-tunable femtosecond laser has been performed to investigate the autofluorescence intensity of major endogenous fluorophores of human skin in dependence on the excitation wavelength. In high-resolution multiphoton images of different skin layers, clear trends were found for fluorophores like keratin, NAD(P)H, melanin as well as for the elastin and collagen networks. The analysis of the measurements is supplemented by additional measurements of fluorescence lifetime imaging and signal-decay curves by time-correlated single-photon counting.

© 2010 OSA

OCIS Codes
(170.1610) Medical optics and biotechnology : Clinical applications
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.5810) Medical optics and biotechnology : Scanning microscopy
(190.1900) Nonlinear optics : Diagnostic applications of nonlinear optics
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: December 17, 2009
Revised Manuscript: February 8, 2010
Manuscript Accepted: February 9, 2010
Published: March 31, 2010

Virtual Issues
Vol. 5, Iss. 8 Virtual Journal for Biomedical Optics

Citation
Hans Georg Breunig, Hauke Studier, and Karsten König, "Multiphoton excitation characteristics of cellular fluorophores of human skin in vivo," Opt. Express 18, 7857-7871 (2010)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-18-8-7857


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