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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 8 — Apr. 12, 2010
  • pp: 8160–8170

Simultaneous imaging of oxygen tension and blood flow in animals using a digital micromirror device

Adrien Ponticorvo and Andrew K. Dunn  »View Author Affiliations

Optics Express, Vol. 18, Issue 8, pp. 8160-8170 (2010)

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In this study we present a novel imaging method that combines high resolution cerebral blood flow imaging with a highly flexible map of absolute pO2. In vivo measurements of pO2 in animals using phosphorescence quenching is a well established method, and is preferable over electrical probes which are inherently invasive and are limited to single point measurements. However, spatially resolved pO2 measurements using phosphorescence lifetime quenching typically require expensive cameras to obtain images of pO2 and often suffer from poor signal to noise. Our approach enables us to retain the high temporal resolution and sensitivity of single point detection of phosphorescence by using a digital micromirror device (DMD) to selectively illuminate arbitrarily shaped regions of tissue. In addition, by simultaneously using Laser Speckle Contrast Imaging (LSCI) to measure relative blood flow, we can better examine the relationship between blood flow and absolute pO2. We successfully used this instrument to study changes that occur during ischemic conditions in the brain with enough spatial resolution to clearly distinguish different regions. This novel instrument will provide researchers with an inexpensive and improved technique to examine multiple hemodynamic parameters simultaneously in the brain as well as other tissues.

© 2010 OSA

OCIS Codes
(110.6150) Imaging systems : Speckle imaging
(170.0110) Medical optics and biotechnology : Imaging systems
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(230.6120) Optical devices : Spatial light modulators

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: October 26, 2009
Revised Manuscript: March 30, 2010
Manuscript Accepted: April 1, 2010
Published: April 2, 2010

Virtual Issues
Vol. 5, Iss. 8 Virtual Journal for Biomedical Optics

Adrien Ponticorvo and Andrew K. Dunn, "Simultaneous imaging of oxygen tension and blood flow in animals using a digital micromirror device," Opt. Express 18, 8160-8170 (2010)

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