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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 8 — Apr. 12, 2010
  • pp: 8491–8498

A simple scanless two-photon fluorescence microscope using selective plane illumination

Jonathan Palero, Susana I.C.O. Santos, David Artigas, and Pablo Loza-Alvarez  »View Author Affiliations

Optics Express, Vol. 18, Issue 8, pp. 8491-8498 (2010)

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We demonstrate a simple scanless two-photon (2p) excited fluorescence microscope based on selective plane illumination microscopy (SPIM). Optical sectioning capability is presented and depth-resolved imaging of cameleon protein in C. elegans pharyngeal muscle is implemented.

© 2010 OSA

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(170.0180) Medical optics and biotechnology : Microscopy
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(190.4180) Nonlinear optics : Multiphoton processes
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:

Original Manuscript: February 26, 2010
Revised Manuscript: March 24, 2010
Manuscript Accepted: March 31, 2010
Published: April 7, 2010

Virtual Issues
Vol. 5, Iss. 8 Virtual Journal for Biomedical Optics

Jonathan Palero, Susana I.C.O. Santos, David Artigas, and Pablo Loza-Alvarez, "A simple scanless two-photon fluorescence microscope using selective plane illumination," Opt. Express 18, 8491-8498 (2010)

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