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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 19, Iss. 1 — Jan. 3, 2011
  • pp: 353–362

Extended depth-of-field microscopic imaging with a variable focus microscope objective

Sheng Liu and Hong Hua  »View Author Affiliations

Optics Express, Vol. 19, Issue 1, pp. 353-362 (2011)

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Increasing the depth-of-field (DOF) while maintaining high resolution imaging has been a classical challenge for conventional microscopes. Extended DOF (EDOF) is especially essential for imaging thick specimens. We present a microscope capable of capturing EDOF images in a single shot. A volumetric optical sampling method is applied by rapidly scanning the focus of a vari-focal microscope objective throughout the extended depths of a thick specimen within the duration of a single detector exposure. An EDOF image is reconstructed by deconvolving the captured image with the response function of the system. Design of a vari-focal objective and algorithms for restoring EDOF images are presented. Proof-of-concept experimental results demonstrate significantly extended DOF compared to the conventional microscope counterparts.

© 2010 OSA

OCIS Codes
(080.3620) Geometric optics : Lens system design
(100.3020) Image processing : Image reconstruction-restoration
(110.4850) Imaging systems : Optical transfer functions
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:

Original Manuscript: September 30, 2010
Revised Manuscript: December 3, 2010
Manuscript Accepted: December 17, 2010
Published: December 23, 2010

Virtual Issues
Vol. 6, Iss. 2 Virtual Journal for Biomedical Optics

Sheng Liu and Hong Hua, "Extended depth-of-field microscopic imaging with a variable focus microscope objective," Opt. Express 19, 353-362 (2011)

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