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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 19, Iss. 1 — Jan. 3, 2011
  • pp: 387–398

Microscope-based label-free microfluidic cytometry

Xuantao Su, Sean E. Kirkwood, Manisha Gupta, Leah Marquez-Curtis, Yuanyuan Qiu, Anna Janowska-Wieczorek, Wojciech Rozmus, and Ying Y. Tsui  »View Author Affiliations

Optics Express, Vol. 19, Issue 1, pp. 387-398 (2011)

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A microscope-based label-free microfluidic cytometer capable of acquiring two dimensional light scatter patterns from single cells, pattern analysis of which determines cellular information such as cell size, orientation and inner nanostructure, was developed. Finite-difference time-domain numerical simulations compared favorably with experimental scatter patterns from micrometer-sized beads and cells. The device was capable of obtaining light scattering patterns from the smallest mature blood cells (platelets) and cord blood hematopoietic stem/progenitor cells (CD34 + cells) and myeloid precursor cells. The potential for evaluation of cells using this label-free microfluidic cytometric technique was discussed.

© 2010 OSA

OCIS Codes
(000.4430) General : Numerical approximation and analysis
(120.0120) Instrumentation, measurement, and metrology : Instrumentation, measurement, and metrology
(120.5820) Instrumentation, measurement, and metrology : Scattering measurements
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.1530) Medical optics and biotechnology : Cell analysis
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(290.0290) Scattering : Scattering

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: September 27, 2010
Revised Manuscript: December 8, 2010
Manuscript Accepted: December 12, 2010
Published: December 24, 2010

Virtual Issues
Vol. 6, Iss. 2 Virtual Journal for Biomedical Optics

Xuantao Su, Sean E. Kirkwood, Manisha Gupta, Leah Marquez-Curtis, Yuanyuan Qiu, Anna Janowska-Wieczorek, Wojciech Rozmus, and Ying Y. Tsui, "Microscope-based label-free microfluidic cytometry," Opt. Express 19, 387-398 (2011)

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