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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 19, Iss. 11 — May. 23, 2011
  • pp: 10063–10072

Simultaneous illumination method: application in a two-channel emission fluorometer with multi-wavelength excitation

Hiroki Ebata, Akihiko Tanaka, Yohei Takahashi, Shintaro Yano, Hiroyuki Tan, Roland Doerffer, and Tomohiko Oishi  »View Author Affiliations


Optics Express, Vol. 19, Issue 11, pp. 10063-10072 (2011)
http://dx.doi.org/10.1364/OE.19.010063


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Abstract

We developed a new illumination method called the simultaneous illumination method. This method does not require synchronization between light sources and sensor signals, which drastically simplifies the instrumentation. As a proof-of-concept, we applied this method to an oceanographic fluorometer. In principle, using this method, one can easily increase the number of characterized emission wavelengths by mounting optical sensors for as many emission wavelengths as needed. Our fluorometer has two emission-wavelength channels and twelve excitation wavelengths. The aim of this prototype is to demonstrate a viable in situ N-channel emission fluorometer with multiple wavelengths of excitation, which has not been previously realized.

© 2011 OSA

OCIS Codes
(010.4450) Atmospheric and oceanic optics : Oceanic optics
(260.2510) Physical optics : Fluorescence
(300.2140) Spectroscopy : Emission
(300.6280) Spectroscopy : Spectroscopy, fluorescence and luminescence
(300.6300) Spectroscopy : Spectroscopy, Fourier transforms

ToC Category:
Spectroscopy

History
Original Manuscript: February 25, 2011
Revised Manuscript: April 30, 2011
Manuscript Accepted: May 2, 2011
Published: May 9, 2011

Virtual Issues
Vol. 6, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Hiroki Ebata, Akihiko Tanaka, Yohei Takahashi, Shintaro Yano, Hiroyuki Tan, Roland Doerffer, and Tomohiko Oishi, "Simultaneous illumination method: application in a two-channel emission fluorometer with multi-wavelength excitation," Opt. Express 19, 10063-10072 (2011)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-19-11-10063


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