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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 19, Iss. 11 — May. 23, 2011
  • pp: 10377–10386

Single-cell isolation using a DVD optical pickup

A. Kasukurti, M. Potcoava, S.A. Desai, C. Eggleton, and D. W. M. Marr  »View Author Affiliations


Optics Express, Vol. 19, Issue 11, pp. 10377-10386 (2011)
http://dx.doi.org/10.1364/OE.19.010377


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Abstract

A low-cost single-cell isolation system incorporating a digital versatile disc burner (DVD RW) optical pickup has been developed. We show that these readily available modules have the required laser power and focusing optics to provide a steady Gaussian beam capable of optically trapping micron-sized colloids and red blood cells. Utility of the pickup is demonstrated through the non-destructive isolation of such particles in a laminar-flow based microfluidic device that captures and translates single microscale objects across streamlines into designated channel exits. In this, the integrated objective lens focusing coils are used to steer the optical trap across the channel, resulting in the isolation of colloids and red blood cells using a very inexpensive off-the-shelf optical component.

© 2011 OSA

OCIS Codes
(140.2020) Lasers and laser optics : Diode lasers
(140.5960) Lasers and laser optics : Semiconductor lasers
(140.7010) Lasers and laser optics : Laser trapping
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.0180) Medical optics and biotechnology : Microscopy
(170.1530) Medical optics and biotechnology : Cell analysis
(170.4520) Medical optics and biotechnology : Optical confinement and manipulation

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: February 23, 2011
Revised Manuscript: April 9, 2011
Manuscript Accepted: April 11, 2011
Published: May 11, 2011

Virtual Issues
Vol. 6, Iss. 6 Virtual Journal for Biomedical Optics

Citation
A. Kasukurti, M. Potcoava, S.A. Desai, C. Eggleton, and D. W. M. Marr, "Single-cell isolation using a DVD optical pickup," Opt. Express 19, 10377-10386 (2011)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-19-11-10377


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