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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 19, Iss. 12 — Jun. 6, 2011
  • pp: 11090–11099

Long range surface plasmon-coupled fluorescence emission for biosensor applications

Koji Toma, Jakub Dostalek, and Wolfgang Knoll  »View Author Affiliations

Optics Express, Vol. 19, Issue 12, pp. 11090-11099 (2011)

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A biosensor scheme that employs long range surface plasmons (LRSPs) for the efficient excitation and collection of fluorescence light from fluorophore-labeled biomolecules captured in a three-dimensional hydrogel matrix is discussed. This new approach to plasmon-enhanced fluorescence (PEF) is experimentally and theoretically investigated by using the Kretschmann configuration of attenuated total reflection (ATR) method. A layer structure supporting LRSPs that consists of a low refractive index fluoropolymer layer, a thin gold film and a large binding capacity N-isopropylacrylamide (NIPAAm)-based hydrogel matrix swollen in an aqueous sample is employed. By using this layer architecture, the extended field of LRSPs probes the binding of biomolecules in the binding matrix at up to micrometer distances from the gold surface. With respect to regular surface plasmon-enhanced fluorescence spectroscopy (SPFS) and surface plasmon-coupled emission (SPCE), a narrower angular distribution of the fluorescence light intensity, a larger peak intensity and the excitation and emission at lower angles were observed.

© 2011 OSA

OCIS Codes
(160.5470) Materials : Polymers
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(240.6680) Optics at surfaces : Surface plasmons

ToC Category:

Original Manuscript: March 18, 2011
Revised Manuscript: May 7, 2011
Manuscript Accepted: May 13, 2011
Published: May 23, 2011

Virtual Issues
Vol. 6, Iss. 7 Virtual Journal for Biomedical Optics

Koji Toma, Jakub Dostalek, and Wolfgang Knoll, "Long range surface plasmon-coupled fluorescence emission for biosensor applications," Opt. Express 19, 11090-11099 (2011)

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