OSA's Digital Library

Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 19, Iss. 12 — Jun. 6, 2011
  • pp: 11732–11739

Enhanced two-photon excited fluorescence in three-dimensionally crosslinked bovine serum albumin microstructures

Keng-Chi Cho, Chi-Hsiang Lien, Chun-Yu Lin, Chia-Yuan Chang, Lynn L. H. Huang, Paul J. Campagnola, Chen Yuan Dong, and Shean-Jen Chen  »View Author Affiliations


Optics Express, Vol. 19, Issue 12, pp. 11732-11739 (2011)
http://dx.doi.org/10.1364/OE.19.011732


View Full Text Article

Enhanced HTML    Acrobat PDF (845 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

In this study, the intensity of two-photon excited fluorescence (TPEF) of xanthene dye, Rose Bengal (RB), was significantly enhanced via bovine serum albumin (BSA) microstructures fabricated by the two-photon crosslinking (TPC) technique. The RB was utilized as the photoactivator in the TPC processing and the enhanced TPEF intensity correlates with the concentration of fabricated crosslinked BSA microstructures via the power control and pulse selection of the employed femtosecond laser. As a result, fabrication of three-dimensional BSA microstructures can be simultaneously monitored by the use of TPEF intensity. The crosslinked BSA microstructures synthesized may be used as an ordered biomaterial for fluorescence enhancement.

© 2011 OSA

OCIS Codes
(160.2540) Materials : Fluorescent and luminescent materials
(190.4180) Nonlinear optics : Multiphoton processes
(220.4000) Optical design and fabrication : Microstructure fabrication

ToC Category:
Materials

History
Original Manuscript: March 16, 2011
Revised Manuscript: May 4, 2011
Manuscript Accepted: May 29, 2011
Published: June 1, 2011

Virtual Issues
Vol. 6, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Keng-Chi Cho, Chi-Hsiang Lien, Chun-Yu Lin, Chia-Yuan Chang, Lynn L. H. Huang, Paul J. Campagnola, Chen Yuan Dong, and Shean-Jen Chen, "Enhanced two-photon excited fluorescence in three-dimensionally crosslinked bovine serum albumin microstructures," Opt. Express 19, 11732-11739 (2011)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-19-12-11732


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. C. R. Lambert, I. E. Kochevar, and R. W. Redmond, “Differential reactivity of upper triplet states produces wavelength-dependent two-photon photosensitization using Rose Bengal,” J. Phys. Chem. B 103(18), 3737–3741 (1999). [CrossRef]
  2. J. D. Pitts, P. J. Campagnola, G. A. Epling, and S. L. Goodman, “Submicron multiphoton free-form fabrication of proteins and polymers: studies of reaction efficiencies and applications in sustained release,” Macromolecules 33(5), 1514–1523 (2000). [CrossRef]
  3. P. J. Campagnola, D. M. Delguidice, G. A. Epling, K. D. Hoffacker, A. R. Howell, J. D. Pitts, and S. L. Goodman, “3-dimensional submicron polymerization of acrylamide by multiphoton excitation of xanthene dyes,” Macromolecules 33(5), 1511–1513 (2000). [CrossRef]
  4. S. Kawata, H. B. Sun, T. Tanaka, and K. Takada, “Finer features for functional microdevices,” Nature 412(6848), 697–698 (2001). [CrossRef] [PubMed]
  5. P. Galajda and P. Ormos, “Complex micromachines produced and driven by light,” Appl. Phys. Lett. 78(2), 249–251 (2001). [CrossRef]
  6. T. Tanaka, H. B. Sun, and S. Kawata, “Rapid sub-diffraction-limit laser micro/nanoprocessing in a threshold material system,” Appl. Phys. Lett. 80(2), 312–314 (2002). [CrossRef]
  7. M. Miwa, S. Juodkazis, T. Kawakami, S. Matsuo, and H. Misawa, “Femtosecond two-photon stereo-lithography,” Appl. Phys., A Mater. Sci. Process. 73(5), 561–566 (2001). [CrossRef]
  8. W. Denk, J. H. Strickler, and W. W. Webb, “Two-photon laser scanning fluorescence microscopy,” Science 248(4951), 73–76 (1990). [CrossRef] [PubMed]
  9. T. Watanabe, M. Akiyama, K. Totani, S. M. Kuebler, F. Stellacci, W. Wenseleers, K. Braun, S. R. Marder, and J. W. Perry, “Photoresponsive hydrogel microstructure fabricated by two-photon initiated Polymerization,” Adv. Funct. Mater. 12(9), 611–614 (2002). [CrossRef]
  10. Z. B. Sun, X. Z. Dong, W. Q. Chen, S. Nakanishi, M. Duan, and S. Kawata, “Multicolor polymer nanocomposites: in situ synthesis and fabrication of 3D microstructures,” Adv. Mater. (Deerfield Beach Fla.) 20(5), 914–919 (2008). [CrossRef]
  11. A. J. Steckl, “DNA – a new material for photonics?” Nat. Photonics 1(1), 3–5 (2007). [CrossRef]
  12. J. A. Hagen, W. Li, A. J. Steckl, and J. G. Grote, “Enhanced emission efficiency in organic light-emitting diodes using deoxyribonucleic acid complex as an electron blocking layer,” Appl. Phys. Lett. 88(17), 171109 (2006). [CrossRef]
  13. Y. Ner, D. Navarathne, D. M. Niedzwiedzki, J. G. Grote, A. V. Dobrynin, H. A. Frank, and G. A. Sotzing, “Stabilization of fluorophore in DNA thin films,” Appl. Phys. Lett. 95(26), 263701 (2009). [CrossRef]
  14. J. A. Hagen, W.-X. Li, H. Spaeth, J. G. Grote, and A. J. Steckl, “Molecular beam deposition of DNA nanometer films,” Nano Lett. 7(1), 133–137 (2007). [CrossRef] [PubMed]
  15. J. Mysliwiec, L. Sznitko, A. Sobolewska, S. Bartkiewicz, and A. Miniewicz, “Lasing effect in a hybrid dye-doped biopolymer and photochromic polymer system,” Appl. Phys. Lett. 96(14), 141106 (2010). [CrossRef]
  16. P. Yang, G. Wirnsberger, H. C. Huang, S. R. Cordero, M. D. McGehee, B. Scott, T. Deng, G. M. Whitesides, B. F. Chmelka, S. K. Buratto, and G. D. Stucky, “Mirrorless lasing from mesostructured waveguides patterned by soft lithography,” Science 287(5452), 465–467 (2000). [CrossRef] [PubMed]
  17. A. Otomo, S. Yokoyama, T. Nakahama, and S. Mashiko, “Supernarrowing mirrorless laser emission in dendrimer-doped polymer waveguides,” Appl. Phys. Lett. 77(24), 3881–3883 (2000). [CrossRef]
  18. D. C. Neckers, “Rose Bengal,” J. Photochem. Photobiol., A 47(1), 1–29 (1989). [CrossRef]
  19. Z. Zhang and T. Yagi, “Observation of group delay dispersion as a function of the pulse width in as mode locked Ti:sapphire laser,” Appl. Phys. Lett. 63(22), 2993–2995 (1993). [CrossRef]
  20. W.-S. Kuo, C.-H. Lien, K.-C. Cho, C.-Y. Chang, C.-Y. Lin, L. L. H. Huang, P. J. Campagnola, C. Y. Dong, and S.-J. Chen, “Multiphoton fabrication of freeform polymer microstructures with gold nanorods,” Opt. Express 18(26), 27550–27559 (2010). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Figures

Fig. 1 Fig. 2 Fig. 3
 
Fig. 4 Fig. 5
 

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited