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Extended focus high-speed swept source OCT with self-reconstructive illuminationCedric Blatter, Branislav Grajciar, Christoph M. Eigenwillig, Wolfgang Wieser, Benjamin R. Biedermann, Robert Huber, and Rainer A. Leitgeb »View Author Affiliations
Cedric Blatter,1
Branislav Grajciar,1
Christoph M. Eigenwillig,2
Wolfgang Wieser,2
Benjamin R. Biedermann,2
Robert Huber,2
and Rainer A. Leitgeb1,*
1Group of Biomedical Optics, Center of Medical Physics and Biomedical Engineering, Medical University Vienna, Waehringerstrasse 13, 1090 Vienna, Austria 2Lehrstuhl für BioMolekulare Optik, Ludwig-Maximilians-Universität München, Oettingenstraße 67, 80538 Munich, Germany *Corresponding author: rainer.leitgeb@meduniwien.ac.at |
Optics Express, Vol. 19, Issue 13, pp. 12141-12155 (2011)
http://dx.doi.org/10.1364/OE.19.012141
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Abstract
We present a Bessel beam illumination FDOCT setup using a FDML Swept Source at 1300nm with up to 440kHz A-scan rate, and discuss its advantages for structural and functional imaging of highly scattering samples. An extended focus is achieved due to the Bessel beam that preserves its lateral extend over a large depth range. Furthermore, Bessel beams exhibit a self-reconstruction property that allows imaging even behind obstacles such as hairs on skin. Decoupling the illumination from the Gaussian detection increases the global sensitivity and enables dark field imaging. Dark field imaging is useful to avoid strong reflexes from the sample surface that adversely affect the sensitivity due to the limited dynamic range of high speed 8bit acquisition cards. In addition the possibility of contrasting capillaries with high sensitivity is shown, using inter-B-scan speckle variance analysis. We demonstrate intrinsic advantages of the extended focus configuration, in particular the reduction of the phase decorrelation effect below vessels leading to improved axial vessel definition.
© 2011 OSA
OCIS Codes
(110.4500) Imaging systems : Optical coherence tomography
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(280.2490) Remote sensing and sensors : Flow diagnostics
(180.1655) Microscopy : Coherence tomography
(170.2655) Medical optics and biotechnology : Functional monitoring and imaging
ToC Category:
Medical Optics and Biotechnology
History
Original Manuscript: March 18, 2011
Revised Manuscript: June 5, 2011
Manuscript Accepted: June 6, 2011
Published: June 8, 2011
Virtual Issues
Vol. 6, Iss. 7 Virtual Journal for Biomedical Optics
Citation
Cedric Blatter, Branislav Grajciar, Christoph M. Eigenwillig, Wolfgang Wieser, Benjamin R. Biedermann, Robert Huber, and Rainer A. Leitgeb, "Extended focus high-speed swept source OCT with self-reconstructive illumination," Opt. Express 19, 12141-12155 (2011)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-19-13-12141
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- R. Leitgeb, C. K. Hitzenberger, and A. F. Fercher, “Performance of fourier domain vs. time domain optical coherence tomography,” Opt. Express 11(8), 889–894 (2003). [CrossRef] [PubMed]
- M. Wojtkowski, R. Leitgeb, A. Kowalczyk, T. Bajraszewski, and A. F. Fercher, “In vivo human retinal imaging by Fourier domain optical coherence tomography,” J. Biomed. Opt. 7(3), 457–463 (2002). [CrossRef] [PubMed]
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- R. Huber, M. Wojtkowski, and J. G. Fujimoto, “Fourier Domain Mode Locking (FDML): A new laser operating regime and applications for optical coherence tomography,” Opt. Express 14(8), 3225–3237 (2006). [CrossRef] [PubMed]
- R. Huber, D. C. Adler, and J. G. Fujimoto, “Buffered Fourier domain mode locking: Unidirectional swept laser sources for optical coherence tomography imaging at 370,000 lines/s,” Opt. Lett. 31(20), 2975–2977 (2006). [CrossRef] [PubMed]
- R. Huber, M. Wojtkowski, J. G. Fujimoto, J. Y. Jiang, and A. E. Cable, “Three-dimensional and C-mode OCT imaging with a compact, frequency swept laser source at 1300 nm,” Opt. Express 13(26), 10523–10538 (2005). [CrossRef] [PubMed]
- B. J. Vakoc, R. M. Lanning, J. A. Tyrrell, T. P. Padera, L. A. Bartlett, T. Stylianopoulos, L. L. Munn, G. J. Tearney, D. Fukumura, R. K. Jain, and B. E. Bouma, “Three-dimensional microscopy of the tumor microenvironment in vivo using optical frequency domain imaging,” Nat. Med. 15(10), 1219–1223 (2009). [CrossRef] [PubMed]
- A. Alex, B. Považay, B. Hofer, S. Popov, C. Glittenberg, S. Binder, and W. Drexler, “Multispectral in vivo three-dimensional optical coherence tomography of human skin,” J. Biomed. Opt. 15(2), 026025 (2010). [CrossRef] [PubMed]
- J. Holmes, S. Hattersley, N. Stone, F. Bazant-Hegemark, and H. Barr, “Multi-channel Fourier domain OCT system with superior lateral resolution for biomedical applications,” Proc. SPIE 6847, 684700 (2008).
- R. Leitgeb, L. Schmetterer, M. Wojtkowski, C. Hitzenberger, M. Sticker, and A. Fercher, “Flow Velocity Measurements by Frequency Domain Short Coherence Interferometry,” Proc. SPIE 4619, 16–21 (2002). [CrossRef]
- M. Pircher, B. Baumann, E. Götzinger, H. Sattmann, and C. K. Hitzenberger, “Simultaneous SLO/OCT imaging of the human retina with axial eye motion correction,” Opt. Express 15(25), 16922–16932 (2007). [CrossRef] [PubMed]
- R. A. Leitgeb, L. Schmetterer, C. K. Hitzenberger, A. F. Fercher, F. Berisha, M. Wojtkowski, and T. Bajraszewski, “Real-time measurement of in vitro flow by Fourier-domain color Doppler optical coherence tomography,” Opt. Lett. 29(2), 171–173 (2004). [CrossRef] [PubMed]
- R. Leitgeb, C. K. Hitzenberger, and A. F. Fercher, “Performance of fourier domain vs. time domain optical coherence tomography,” Opt. Express 11(8), 889–894 (2003). [CrossRef] [PubMed]
- A. F. Fercher, C. K. Hitzenberger, G. Kamp, and S. Y. El-Zaiat, “Measurement of intraocular distances by backscattering spectral interferometry,” Opt. Commun. 117(1-2), 43–48 (1995). [CrossRef]
- A. Alex, B. Považay, B. Hofer, S. Popov, C. Glittenberg, S. Binder, and W. Drexler, “Multispectral in vivo three-dimensional optical coherence tomography of human skin,” J. Biomed. Opt. 15(2), 026025 (2010). [CrossRef] [PubMed]
- J. Holmes, S. Hattersley, N. Stone, F. Bazant-Hegemark, and H. Barr, “Multi-channel Fourier domain OCT system with superior lateral resolution for biomedical applications,” Proc. SPIE 6847, 684700 (2008).
- W. Wieser, B. R. Biedermann, T. Klein, C. M. Eigenwillig, and R. Huber, “Multi-megahertz OCT: High quality 3D imaging at 20 million A-scans and 4.5 GVoxels per second,” Opt. Express 18(14), 14685–14704 (2010). [CrossRef] [PubMed]
- R. Huber, D. C. Adler, and J. G. Fujimoto, “Buffered Fourier domain mode locking: Unidirectional swept laser sources for optical coherence tomography imaging at 370,000 lines/s,” Opt. Lett. 31(20), 2975–2977 (2006). [CrossRef] [PubMed]
- R. Huber, M. Wojtkowski, and J. G. Fujimoto, “Fourier Domain Mode Locking (FDML): A new laser operating regime and applications for optical coherence tomography,” Opt. Express 14(8), 3225–3237 (2006). [CrossRef] [PubMed]
- R. Huber, M. Wojtkowski, J. G. Fujimoto, J. Y. Jiang, and A. E. Cable, “Three-dimensional and C-mode OCT imaging with a compact, frequency swept laser source at 1300 nm,” Opt. Express 13(26), 10523–10538 (2005). [CrossRef] [PubMed]
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