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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 19, Iss. 13 — Jun. 20, 2011
  • pp: 12605–12618

Multispectral Cerenkov luminescence tomography for small animal optical imaging

Antonello E Spinelli, Chaincy Kuo, Brad W Rice, Riccardo Calandrino, Pasquina Marzola, Andrea Sbarbati, and Federico Boschi  »View Author Affiliations

Optics Express, Vol. 19, Issue 13, pp. 12605-12618 (2011)

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Quite recently Cerenkov luminescence imaging (CLI) has been introduced as a novel pre-clinical imaging for the in vivo imaging of small animals such as mice. The CLI method is based on the detection of Cerenkov radiation (CR) generated by beta particles as they travel into the animal tissues with an energy such that Cerenkov emission condition is satisfied. This paper describes an image reconstruction method called multi spectral diffuse Cerenkov luminescence tomography (msCLT) in order to obtain 3D images from the detection of CR. The multispectral approach is based on a set of 2D planar images acquired using a number of narrow bandpass filters, and the distinctive information content at each wavelength is used in the 3D image reconstruction process. The proposed msCLT method was tested both in vitro and in vivo using 32P-ATP and all the images were acquired by using the IVIS 200 small animal optical imager (Caliper Life Sciences, Alameda USA). Source depth estimation and spatial resolution measurements were performed using a small capillary source placed between several slices of chicken breast. The theoretical Cerenkov emission spectrum and optical properties of chicken breast were used in the modelling of photon propagation. In vivo imaging was performed by injecting control nude mice with 10 MBq of 32P-ATP and the 3D tracer bio-distribution was reconstructed. Whole body MRI was acquired to provide an anatomical localization of the Cerenkov emission. The spatial resolution obtained from the msCLT reconstructed images of the capillary source showed that the FWHM is about 1.5 mm for a 6 mm depth. Co-registered MRI images showed that the Cerenkov emission regions matches fairly well with anatomical regions, such as the brain, heart and abdomen. Ex vivo imaging of the different organs such as intestine, brain, heart and ribs further confirms these findings. We conclude that in vivo 3D bio-distribution of a pure beta-minus emitting radiopharmaceutical such as 32P-ATP can be obtained using the msCLT reconstruction approach.

© 2011 OSA

OCIS Codes
(100.0100) Image processing : Image processing
(100.3010) Image processing : Image reconstruction techniques

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: April 19, 2011
Revised Manuscript: June 8, 2011
Manuscript Accepted: June 8, 2011
Published: June 14, 2011

Virtual Issues
Vol. 6, Iss. 7 Virtual Journal for Biomedical Optics

Antonello E Spinelli, Chaincy Kuo, Brad W Rice, Riccardo Calandrino, Pasquina Marzola, Andrea Sbarbati, and Federico Boschi, "Multispectral Cerenkov luminescence tomography for small animal optical imaging," Opt. Express 19, 12605-12618 (2011)

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