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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 19, Iss. 15 — Jul. 18, 2011
  • pp: 13839–13847

High-speed 2D and 3D fluorescence microscopy of cardiac myocytes

Sunil Kumar, Dean Wilding, Markus B. Sikkel, Alexander R. Lyon, Ken T. MacLeod, and Chris Dunsby  »View Author Affiliations


Optics Express, Vol. 19, Issue 15, pp. 13839-13847 (2011)
http://dx.doi.org/10.1364/OE.19.013839


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Abstract

Oblique plane microscopy (OPM) is a light sheet microscopy technique that uses a single high numerical aperture microscope objective to both illuminate a tilted plane within the specimen and to obtain an image of the tilted illuminated plane. In this paper, we present a new OPM configuration that enables both the illumination and detection focal planes to be swept simultaneously and remotely through the sample volume, enabling high speed volumetric imaging. We demonstrate the high speed imaging capabilities of the system by imaging calcium dynamics in cardiac myocytes in 2D at 926 frames per second and in 3D at 21 volumes per second. In the future, higher frame rate CCD cameras will enable volumetric imaging at much greater rates, leading to new capabilities to study dynamic events in cells at high speeds in two and three dimensions.

© 2011 OSA

OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Microscopy

History
Original Manuscript: March 16, 2011
Revised Manuscript: May 14, 2011
Manuscript Accepted: May 16, 2011
Published: July 6, 2011

Virtual Issues
Vol. 6, Iss. 8 Virtual Journal for Biomedical Optics

Citation
Sunil Kumar, Dean Wilding, Markus B. Sikkel, Alexander R. Lyon, Ken T. MacLeod, and Chris Dunsby, "High-speed 2D and 3D fluorescence microscopy of cardiac myocytes," Opt. Express 19, 13839-13847 (2011)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-19-15-13839


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