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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 19, Iss. 15 — Jul. 18, 2011
  • pp: 14172–14181

Microlensed dual-fiber probe for depth-resolved fluorescence measurements

Hae Young Choi, Seon Young Ryu, Jae Young Kim, Geon Hee Kim, Seong Jun Park, Byeong Ha Lee, and Ki Soo Chang  »View Author Affiliations


Optics Express, Vol. 19, Issue 15, pp. 14172-14181 (2011)
http://dx.doi.org/10.1364/OE.19.014172


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Abstract

We propose and demonstrate a compact microlensed dual-fiber probe that has a good collection efficiency and a high depth-resolution ability for fluorescence measurements. The probe is formed with a conventional fusion splicer creating a common focusing lens on two fibers placed side by side. The collection efficiency of the fabricated probe was evaluated by measuring the fluorescence signal of a fresh ginkgo leaf. It was shown experimentally that the proposed probe could effectively collect the fluorescence signal with a six-fold increase compared to that of a general flat-tipped probe. The beam propagation method was used to design a probe with an optimized working distance and an improved resolving depth. It was found that the working distance depends mainly on the radius of curvature of the lens, whereas the resolving depth is determined by the core diameters of the illumination and collection fibers. The depth-resolved ability of probes with working distances of ~100 μm and 300 μm was validated by using a two-layer tissue phantom. The experimental results demonstrate that the microlensed dual-fiber probe has the potential to facilitate depth-resolved fluorescence detection of epithelial tissue.

© 2011 OSA

OCIS Codes
(060.2370) Fiber optics and optical communications : Fiber optics sensors
(120.4570) Instrumentation, measurement, and metrology : Optical design of instruments
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(350.3950) Other areas of optics : Micro-optics

ToC Category:
Instrumentation, Measurement, and Metrology

History
Original Manuscript: April 25, 2011
Revised Manuscript: June 20, 2011
Manuscript Accepted: June 23, 2011
Published: July 11, 2011

Virtual Issues
Vol. 6, Iss. 8 Virtual Journal for Biomedical Optics

Citation
Hae Young Choi, Seon Young Ryu, Jae Young Kim, Geon Hee Kim, Seong Jun Park, Byeong Ha Lee, and Ki Soo Chang, "Microlensed dual-fiber probe for depth-resolved fluorescence measurements," Opt. Express 19, 14172-14181 (2011)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-19-15-14172


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