OSA's Digital Library

Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 19, Iss. 16 — Aug. 1, 2011
  • pp: 15062–15068

Polarization state studies in second harmonic generation signals to trace atherosclerosis lesions

Camille Doras, Gregory Taupier, Alberto Barsella, Loïc Mager, Alex Boeglin, Hervé Bulou, Pascal Bousquet, and Kokou D. Dorkenoo  »View Author Affiliations


Optics Express, Vol. 19, Issue 16, pp. 15062-15068 (2011)
http://dx.doi.org/10.1364/OE.19.015062


View Full Text Article

Enhanced HTML    Acrobat PDF (1293 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We have performed multi-photon image reconstructions as well as polarization state analyses inside an artery wall affected by atherosclerosis to investigate the changes in collagen structure. Mice, either healthy or affected by spontaneous atherosclerosis, have been used for this purpose. A two-photon imaging system has been used to investigate atherosclerotic lesions in the ascending aorta of mice. Second harmonic imaging has been performed alternatively on healthy samples and on affected region. The reconstructed images show that the spatial distribution of the collagen network seems disorganized by the disease. The polarization state studies reveal however that the apparent disorganization of the collagen is related to its spatially diffuse distribution and that the internal structure of the collagen fibers is not affected by the disease. In addition, a theoretical simulation of the second harmonic polarization states shows that they are consistent with the known 3D structure of the collagen network.

© 2011 OSA

OCIS Codes
(190.1900) Nonlinear optics : Diagnostic applications of nonlinear optics
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: April 4, 2011
Revised Manuscript: June 30, 2011
Manuscript Accepted: June 30, 2011
Published: July 21, 2011

Virtual Issues
Vol. 6, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Camille Doras, Gregory Taupier, Alberto Barsella, Loïc Mager, Alex Boeglin, Hervé Bulou, Pascal Bousquet, and Kokou D. Dorkenoo, "Polarization state studies in second harmonic generation signals to trace atherosclerosis lesions," Opt. Express 19, 15062-15068 (2011)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-19-16-15062


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. S. Roth and I. Freund, “Second-harmonic generation in collagen,” J. Chem. Phys. 70(4), 1637–1643 (1979). [CrossRef]
  2. P. J. Campagnola, A. C. Millard, M. Terasaki, P. E. Hoppe, C. J. Malone, and W. A. Mohler, “Three-dimensional high-resolution second-harmonic generation imaging of endogenous structural proteins in biological tissues,” Biophys. J. 82(1), 493–508 (2002). [CrossRef] [PubMed]
  3. R. M. Williams, W. R. Zipfel, and W. W. Webb, “Interpreting second-harmonic generation images of collagen I fibrils,” Biophys. J. 88(2), 1377–1386 (2005). [CrossRef] [PubMed]
  4. M. Strupler, A. M. Pena, M. Hernest, P. L. Tharaux, J. L. Martin, E. Beaurepaire, and M. C. Schanne-Klein, “Second harmonic imaging and scoring of collagen in fibrotic tissues,” Opt. Express 15(7), 4054–4065 (2007). [CrossRef] [PubMed]
  5. W. R. Zipfel, R. M. Williams, R. Christie, A. Y. Nikitin, B. T. Hyman, and W. W. Webb, “Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation,” Proc. Natl. Acad. Sci. U.S.A. 100(12), 7075–7080 (2003). [CrossRef] [PubMed]
  6. S. W. Chu, S. P. Tai, M. C. Chan, C. K. Sun, I. C. Hsiao, C. H. Lin, Y. C. Chen, and B. L. Lin, “Thickness dependence of optical second harmonic generation in collagen fibrils,” Opt. Express 15(19), 12005–12010 (2007). [CrossRef] [PubMed]
  7. S. Brasselet, D. Ait-Belkacem, A. Gasecka, F. Munhoz, S. Brustlein, and S. Brasselet, “Influence of birefringence on polarization resolved nonlinear microscopy and collagen SHG structural imaging,” Opt. Express 18(14), 14859–14870 (2010). [CrossRef] [PubMed]
  8. I. Gusachenko, G. Latour, and M. C. Schanne-Klein, “Polarization-resolved Second Harmonic microscopy in anisotropic thick tissues,” Opt. Express 18(18), 19339–19352 (2010). [CrossRef] [PubMed]
  9. P. J. Su, W. L. Chen, J. B. Hong, T. H. Li, R. J. Wu, C. K. Chou, S. J. Chen, C. Hu, S. J. Lin, and C. Y. Dong, “Discrimination of collagen in normal and pathological skin dermis through second-order susceptibility microscopy,” Opt. Express 17(13), 11161–11171 (2009). [CrossRef] [PubMed]
  10. A. Zoumi, X. Lu, G. S. Kassab, and B. J. Tromberg, “Imaging coronary artery microstructure using second-harmonic and two-photon fluorescence microscopy,” Biophys. J. 87(4), 2778–2786 (2004). [CrossRef] [PubMed]
  11. T. Boulesteix, A. M. Pena, N. Pages, G. Godeau, M. P. Sauviat, E. Beaurepaire, and M. C. Schanne-Klein, “Micrometer scale ex vivo multiphoton imaging of unstained arterial wall structure,” Cytometry A 69(1), 20–26 (2006). [CrossRef] [PubMed]
  12. T. T. Le, I. M. Langohr, M. J. Locker, M. Sturek, and J. X. Cheng, “Label-free molecular imaging of atherosclerotic lesions using multimodal nonlinear optical microscopy,” J. Biomed. Opt. 12(5), 054007 (2007). [CrossRef] [PubMed]
  13. H. W. Wang, I. M. Langohr, M. Sturek, and J. X. Cheng, “Imaging and quantitative analysis of atherosclerotic lesions by CARS-based multimodal nonlinear optical microscopy,” Arterioscler. Thromb. Vasc. Biol. 29(9), 1342–1348 (2009). [CrossRef] [PubMed]
  14. R. S. Lim, A. Kratzer, N. P. Barry, S. Miyazaki-Anzai, M. Miyazaki, W. W. Mantulin, M. Levi, E. O. Potma, and B. J. Tromberg, “Multimodal CARS microscopy determination of the impact of diet on macrophage infiltration and lipid accumulation on plaque formation in ApoE-deficient mice,” J. Lipid Res. 51(7), 1729–1737 (2010). [CrossRef] [PubMed]
  15. P. A. Franken, A. E. Hill, C. W. Peters, and G. W. Weinreich, “Generation of optical harmonics,” Phys. Rev. Lett. 7(4), 118–119 (1961). [CrossRef]
  16. D. A. Kleinman, “Theory of second harmonic generation of light,” Phys. Rev. 128(4), 1761–1775 (1962). [CrossRef]
  17. S. Roth and I. Freund, “Second harmonic generation and orientational order in connective tissue: a mosaic model for fibril orientational ordering in rat-tail tendon,” J. Appl. Cryst. 15(1), 72–78 (1982). [CrossRef]
  18. I. Freund, M. Deutsch, and A. Sprecher, “Connective tissue polarity. Optical second-harmonic microscopy, crossed-beam summation, and small-angle scattering in rat-tail tendon,” Biophys. J. 50(4), 693–712 (1986). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited