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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 19, Iss. 16 — Aug. 1, 2011
  • pp: 15062–15068

Polarization state studies in second harmonic generation signals to trace atherosclerosis lesions

Camille Doras, Gregory Taupier, Alberto Barsella, Loïc Mager, Alex Boeglin, Hervé Bulou, Pascal Bousquet, and Kokou D. Dorkenoo  »View Author Affiliations

Optics Express, Vol. 19, Issue 16, pp. 15062-15068 (2011)

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We have performed multi-photon image reconstructions as well as polarization state analyses inside an artery wall affected by atherosclerosis to investigate the changes in collagen structure. Mice, either healthy or affected by spontaneous atherosclerosis, have been used for this purpose. A two-photon imaging system has been used to investigate atherosclerotic lesions in the ascending aorta of mice. Second harmonic imaging has been performed alternatively on healthy samples and on affected region. The reconstructed images show that the spatial distribution of the collagen network seems disorganized by the disease. The polarization state studies reveal however that the apparent disorganization of the collagen is related to its spatially diffuse distribution and that the internal structure of the collagen fibers is not affected by the disease. In addition, a theoretical simulation of the second harmonic polarization states shows that they are consistent with the known 3D structure of the collagen network.

© 2011 OSA

OCIS Codes
(190.1900) Nonlinear optics : Diagnostic applications of nonlinear optics
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: April 4, 2011
Revised Manuscript: June 30, 2011
Manuscript Accepted: June 30, 2011
Published: July 21, 2011

Virtual Issues
Vol. 6, Iss. 9 Virtual Journal for Biomedical Optics

Camille Doras, Gregory Taupier, Alberto Barsella, Loïc Mager, Alex Boeglin, Hervé Bulou, Pascal Bousquet, and Kokou D. Dorkenoo, "Polarization state studies in second harmonic generation signals to trace atherosclerosis lesions," Opt. Express 19, 15062-15068 (2011)

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