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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 19, Iss. 17 — Aug. 15, 2011
  • pp: 16410–16417

Holographic deconvolution microscopy for high-resolution particle tracking

Lisa Dixon, Fook Chiong Cheong, and David G. Grier  »View Author Affiliations

Optics Express, Vol. 19, Issue 17, pp. 16410-16417 (2011)

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Rayleigh-Sommerfeld back-propagation can be used to reconstruct the three-dimensional light field responsible for the recorded intensity in an in-line hologram. Deconvolving the volumetric reconstruction with an optimal kernel derived from the Rayleigh-Sommerfeld propagator itself emphasizes the objects responsible for the scattering pattern while suppressing both the propagating light and also such artifacts as the twin image. Bright features in the deconvolved volume may be identified with such objects as colloidal spheres and nanorods. Tracking their thermally-driven Brownian motion through multiple holographic video images provides estimates of the tracking resolution, which approaches 1 nm in all three dimensions.

© 2011 OSA

OCIS Codes
(100.2960) Image processing : Image analysis
(180.6900) Microscopy : Three-dimensional microscopy
(350.4990) Other areas of optics : Particles
(090.1995) Holography : Digital holography

ToC Category:

Original Manuscript: May 16, 2011
Revised Manuscript: July 19, 2011
Manuscript Accepted: August 1, 2011
Published: August 11, 2011

Virtual Issues
Vol. 6, Iss. 9 Virtual Journal for Biomedical Optics

Lisa Dixon, Fook Chiong Cheong, and David G. Grier, "Holographic deconvolution microscopy for high-resolution particle tracking," Opt. Express 19, 16410-16417 (2011)

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