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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 19, Iss. 18 — Aug. 29, 2011
  • pp: 17143–17150

In vivo near-realtime volumetric optical-resolution photoacoustic microscopy using a high-repetition-rate nanosecond fiber-laser

Wei Shi, Parsin Hajireza, Peng Shao, Alexander Forbrich, and Roger J. Zemp  »View Author Affiliations


Optics Express, Vol. 19, Issue 18, pp. 17143-17150 (2011)
http://dx.doi.org/10.1364/OE.19.017143


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Abstract

Optical-resolution photoacoustic microscopy (OR-PAM) is capable of achieving optical-absorption-contrast images with micron-scale spatial resolution. Previous OR-PAM systems have been frame-rate limited by mechanical scanning speeds and laser pulse repetition rate (PRR). We demonstrate OR-PAM imaging using a diode-pumped nanosecond-pulsed Ytterbium-doped 532-nm fiber laser with PRR up to 600 kHz. Combined with fast-scanning mirrors, our proposed system provides C-scan and 3D images with acquisition frame rate of 4 frames per second (fps) or higher, two orders of magnitude faster than previously published systems. High-contrast images of capillary-scale microvasculature in a live Swiss Webster mouse ear with ~6-µm optical lateral spatial resolution are demonstrated.

© 2011 OSA

OCIS Codes
(140.3510) Lasers and laser optics : Lasers, fiber
(170.5120) Medical optics and biotechnology : Photoacoustic imaging
(170.5810) Medical optics and biotechnology : Scanning microscopy

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: June 13, 2011
Revised Manuscript: July 12, 2011
Manuscript Accepted: July 16, 2011
Published: August 17, 2011

Virtual Issues
Vol. 6, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Wei Shi, Parsin Hajireza, Peng Shao, Alexander Forbrich, and Roger J. Zemp, "In vivo near-realtime volumetric optical-resolution photoacoustic microscopy using a high-repetition-rate nanosecond fiber-laser," Opt. Express 19, 17143-17150 (2011)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-19-18-17143


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