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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 19, Iss. 18 — Aug. 29, 2011
  • pp: 17318–17335

Reconstructing an icosahedral virus from single-particle diffraction experiments

D. K. Saldin, H.-C. Poon, P. Schwander, M. Uddin, and M. Schmidt  »View Author Affiliations

Optics Express, Vol. 19, Issue 18, pp. 17318-17335 (2011)

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The first experimental data from single-particle scattering experiments from free electron lasers (FELs) are now becoming available. The first such experiments are being performed on relatively large objects such as viruses, which produce relatively low-resolution, low-noise diffraction patterns in so-called “diffract-and-destroy” experiments. We describe a very simple test on the angular correlations of measured diffraction data to determine if the scattering is from an icosahedral particle. If this is confirmed, the efficient algorithm proposed can then combine diffraction data from multiple shots of particles in random unknown orientations to generate a full 3D image of the icosahedral particle. We demonstrate this with a simulation for the satellite tobacco necrosis virus (STNV), the atomic coordinates of whose asymmetric unit is given in Protein Data Bank entry 2BUK.

© 2011 OSA

OCIS Codes
(100.3200) Image processing : Inverse scattering

ToC Category:
Image Processing

Original Manuscript: June 7, 2011
Revised Manuscript: July 24, 2011
Manuscript Accepted: July 27, 2011
Published: August 18, 2011

Virtual Issues
Vol. 6, Iss. 9 Virtual Journal for Biomedical Optics

D. K. Saldin, H.-C. Poon, P. Schwander, M. Uddin, and M. Schmidt, "Reconstructing an icosahedral virus from single-particle diffraction experiments," Opt. Express 19, 17318-17335 (2011)

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