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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 19, Iss. 18 — Aug. 29, 2011
  • pp: 17439–17452

Depth-resolved image mapping spectrometer (IMS) with structured illumination

Liang Gao, Noah Bedard, Nathan Hagen, Robert T. Kester, and Tomasz S. Tkaczyk  »View Author Affiliations

Optics Express, Vol. 19, Issue 18, pp. 17439-17452 (2011)

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We present a depth-resolved Image Mapping Spectrometer (IMS) which is capable of acquiring 4D (x, y, z, λ) datacubes. Optical sectioning is implemented by structured illumination. The device’s spectral imaging performance is demonstrated in a multispectral microsphere and mouse kidney tissue fluorescence imaging experiment. We also compare quantitatively the depth-resolved IMS with a hyperspectral confocal microscope (HCM) in a standard fluorescent bead imaging experiment. The comparison results show that despite the use of a light source with four orders of magnitude lower intensity in the IMS than that in the HCM, the image signal-to-noise ratio acquired by the IMS is 2.6 times higher than that achieved by the equivalent confocal approach.

© 2011 OSA

OCIS Codes
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(180.2520) Microscopy : Fluorescence microscopy
(110.4234) Imaging systems : Multispectral and hyperspectral imaging

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: July 11, 2011
Revised Manuscript: August 17, 2011
Manuscript Accepted: August 18, 2011
Published: August 19, 2011

Virtual Issues
Vol. 6, Iss. 9 Virtual Journal for Biomedical Optics

Liang Gao, Noah Bedard, Nathan Hagen, Robert T. Kester, and Tomasz S. Tkaczyk, "Depth-resolved image mapping spectrometer (IMS) with structured illumination," Opt. Express 19, 17439-17452 (2011)

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