OSA's Digital Library

Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 19, Iss. 20 — Sep. 26, 2011
  • pp: 18965–18978

Multiplex spectral surface plasmon resonance imaging (SPRI) sensor based on the polarization control scheme

Chi Lok Wong, George Chung Kit Chen, Beng Koon Ng, Shuchi Agarwal, Zhiping Lin, Peng Chen, and Ho Pui Ho  »View Author Affiliations

Optics Express, Vol. 19, Issue 20, pp. 18965-18978 (2011)

View Full Text Article

Enhanced HTML    Acrobat PDF (1164 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



A two-dimensional (2D) spectral SPR sensor based on a polarization control scheme is reported in this paper. The polarization control configuration converts the phase difference between p- and s- polarization occurring at surface plasmon resonance (SPR) into corresponding color responses in spectral SPR images. A sensor resolution of 2.7 x 10−6 RIU has been demonstrated, which corresponds to more than one order of magnitude resolution improvement (26 times) comparing to existing 2D spectral SPR sensors. Multiplex array detection has also been demonstrated with the spectral SPR imaging sensor. In a 8 x 4 sensor array, 32 samples with different refractive index values were monitored simultaneously. Detection on bovine serum albumin (BSA) antigen-antibody binding further demonstrated the multiplex detection capability of the 2D spectral SPR sensor for bio-molecular interactions. The detection limit is found to be 21ng/ml, which is 36 times better than the detection limit previously reported by phase imaging SPR sensors. In light of the advantages of high sensitivity, 2D multiplex detection and real-time response, the spectral SPR imaging sensor can find promising applications in rapid, high throughput, non-labeling and multiplex detection of protein array for proteomics studies, biomarker screening, disease prognosis, and drug discovery.

© 2011 OSA

OCIS Codes
(120.5050) Instrumentation, measurement, and metrology : Phase measurement
(240.6680) Optics at surfaces : Surface plasmons
(260.5430) Physical optics : Polarization
(300.6490) Spectroscopy : Spectroscopy, surface
(280.4788) Remote sensing and sensors : Optical sensing and sensors

ToC Category:

Original Manuscript: March 24, 2011
Revised Manuscript: April 25, 2011
Manuscript Accepted: April 25, 2011
Published: September 15, 2011

Virtual Issues
Vol. 6, Iss. 10 Virtual Journal for Biomedical Optics

Chi Lok Wong, George Chung Kit Chen, Beng Koon Ng, Shuchi Agarwal, Zhiping Lin, Peng Chen, and Ho Pui Ho, "Multiplex spectral surface plasmon resonance imaging (SPRI) sensor based on the polarization control scheme," Opt. Express 19, 18965-18978 (2011)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. M. Srivastava, O. Eidelman, C. Jozwik, C. Paweletz, W. Huang, P. L. Zeitlin, and H. B. Pollard, “Serum proteomic signature for cystic fibrosis using an antibody microarray platform,” Mol. Genet. Metab. 87(4), 303–310 (2006). [CrossRef] [PubMed]
  2. G. Mor, I. Visintin, Y. Lai, H. Zhao, P. Schwartz, T. Rutherford, L. Yue, P. Bray-Ward, and D. C. Ward, “Serum protein markers for early detection of ovarian cancer,” Proc. Natl. Acad. Sci. U.S.A. 102(21), 7677–7682 (2005). [CrossRef] [PubMed]
  3. M. A. Reynolds, H. J. Kirchick, J. R. Dahlen, J. M. Anderberg, P. H. McPherson, K. K. Nakamura, D. T. Laskowitz, G. E. Valkirs, and K. F. Buechler, “Early biomarkers of stroke,” Clin. Chem. 49(10), 1733–1739 (2003). [CrossRef] [PubMed]
  4. P. Angenendt, “Progress in protein and antibody microarray technology,” Drug Discov. Today 10(7), 503–511 (2005). [CrossRef] [PubMed]
  5. B. B. Haab, “Methods and applications of antibody microarrays in cancer research,” Proteomics 3(11), 2116–2122 (2003). [CrossRef] [PubMed]
  6. R. P. Huang, R. Huang, Y. Fan, and Y. Lin, “Simultaneous detection of multiple cytokines from conditioned media and patient’s sera by an antibody-based protein array system,” Anal. Biochem. 294(1), 55–62 (2001). [CrossRef] [PubMed]
  7. G. MacBeath and S. L. Schreiber, “Printing proteins as microarrays for high-throughput function determination,” Science 289(5485), 1760–1763 (2000). [PubMed]
  8. T. O. Joos, M. Schrenk, P. Höpfl, K. Kröger, U. Chowdhury, D. Stoll, D. Schörner, M. Dürr, K. Herick, S. Rupp, K. Sohn, and H. Hämmerle, “A microarray enzyme-linked immunosorbent assay for autoimmune diagnostics,” Electrophoresis 21(13), 2641–2650 (2000). [CrossRef] [PubMed]
  9. H. Ge, “UPA, a universal protein array system for quantitative detection of protein-protein, protein-DNA, protein-RNA and protein-ligand interactions,” Nucleic Acids Res. 28(2), 3e (2000). [CrossRef] [PubMed]
  10. C. L. Wong, H. P. Ho, T. T. Yu, Y. K. Suen, W. W. Y. Chow, S. Y. Wu, W. C. Law, W. Yuan, W. J. Li, S. K. Kong, and C. Lin, “Two-dimensional biosensor arrays based on surface plasmon resonance phase imaging,” Appl. Opt. 46(12), 2325–2332 (2007). [CrossRef] [PubMed]
  11. C. L. Wong, H. P. Ho, Y. K. Suen, S. K. Kong, Q. L. Chen, W. Yuan, and S. Y. Wu, “Real-time protein biosensor arrays based on surface plasmon resonance differential phase imaging,” Biosens. Bioelectron. 24(4), 606–612 (2008). [CrossRef] [PubMed]
  12. J. Homola, S. S. Yee, and G. Gauglitz, “Surface plasmon resonance sensors: review,” Sens. Actuators B Chem. 54(1-2), 3–15 (1999). [CrossRef]
  13. J. Homola, Surface Plasmon Resonance Based Sensors (Springer-Verlag, 2006).
  14. J. Homola, H. Vaisocherová, J. Dostálek, and M. Piliarik, “Multi-analyte surface plasmon resonance biosensing,” Methods 37(1), 26–36 (2005). [CrossRef] [PubMed]
  15. J. S. Yuk, S. J. Yi, H. G. Lee, H. J. Lee, Y. M. Kim, and K. S. Ha, “Characterization of surface plasmon resonance wavelength by changes of protein concentration on protein chips,” Sens. Actuators B Chem. 94(2), 161–164 (2003). [CrossRef]
  16. J. S. Yuk, H. S. Kim, J. W. Jung, S. H. Jung, S. J. Lee, W. J. Kim, J. A. Han, Y. M. Kim, and K. S. Ha, “Analysis of protein interactions on protein arrays by a novel spectral surface plasmon resonance imaging,” Biosens. Bioelectron. 21(8), 1521–1528 (2006). [CrossRef] [PubMed]
  17. J. S. Yuk, J. W. Jung, Y. M. Kim, and K. S. Ha, “Analysis of protein arrays with a dual-function SPR biosensor composed of surface plasmon microscopy and SPR spectroscopy based on white light,” Sens. Actuators B Chem. 129(1), 113–119 (2008). [CrossRef]
  18. J. S. Yuk, D. G. Hong, H. I. Jung, and K. S. Ha, “Application of spectral SPR imaging for the surface analysis of C-reactive protein binding,” Sens. Actuators B Chem. 119(2), 673–675 (2006). [CrossRef]
  19. J. S. Yuk, M. J. Lee, U. R. Kim, and K. S. Ha, “Analysis of blood proteins on protein arrays with a spectral surface plasmon resonance biosensor,” Curr. Appl. Phys. 7(1), 102–107 (2007). [CrossRef]
  20. J. S. Yuk, J. W. Jung, S. H. Jung, J. A. Han, Y. M. Kim, and K. S. Ha, “Sensitivity of ex situ and in situ spectral surface plasmon resonance sensors in the analysis of protein arrays,” Biosens. Bioelectron. 20(11), 2189–2196 (2005). [CrossRef] [PubMed]
  21. J. S. Yuk, J. W. Jung, J. Hyun, Y. M. Kim, and K. S. Ha, “Development of a scanning surface plasmon microscope based on white light for analysis of a wide range of protein arrays,” Sens. Actuators B Chem. 131(1), 241–246 (2008). [CrossRef]
  22. H. P. Ho, C. L. Wong, K. S. Chan, S. Y. Wu, and C. Lin, “Application of two-dimensional spectral surface plasmon resonance to imaging of pressure distribution in elastohydrodynamic lubricant films,” Appl. Opt. 45(23), 5819–5826 (2006). [CrossRef] [PubMed]
  23. C. L. Wong, H. P. Ho, K. S. Chan, P. L. Wong, S. Y. Wu, F. Guo, and C. Lin, “Application of 2-D spectral surface plasmon resonance imaging to studying elastohydrodynamic lubricant (EHL) films,” Tribol. Int. 41(5), 356–366 (2008). [CrossRef]
  24. C. L. Wong, H. P. Ho, K. S. Chan, and S. Y. Wu, “Application of surface plasmon resonance sensing to studying elastohydrodynamic lubricant films,” Appl. Opt. 44(23), 4830–4837 (2005). [CrossRef] [PubMed]
  25. A. Otto, “Excitation of nonradiative surface plasma waves in silver by the method of frustrated total reflection,” Z. Phys. 216(4), 398–410 (1968). [CrossRef]
  26. E. Kretschmann, “The determination of the optical constants of metals by the excitation of surface plasmons,” Z. Phys. 241, 313 (1974).
  27. C. L. Wong, Imaging surface plasmon resonance (SPR) photonic sensors, Thesis (Ph.D.), The City University of Hong Kong, 2007.
  28. S. G. Nelson, K. S. Johnston, and S. S. Yee, “High sensitivity surface plasmon resonance sensor based on phase detection,” Sens. Actuators B Chem. 35(1-3), 187–191 (1996). [CrossRef]
  29. P. Yeh, Optical waves in layered media, (Wiley, 1988).
  30. S. Y. Wu, H. P. Ho, W. C. Law, C. Lin, and S. K. Kong, “Highly sensitive differential phase-sensitive surface plasmon resonance biosensor based on the Mach-Zehnder configuration,” Opt. Lett. 29(20), 2378–2380 (2004). [CrossRef] [PubMed]
  31. H. Eugene, Optics, 4th ed. (Addison-Wesley Publishing Company, 2001).
  32. J. Homola and S. S. Yee, “Novel polarization control scheme for spectral surface plasmon resonance sensors,” Sens. Actuators B Chem. 51(1–3), 331–339 (1998). [CrossRef]
  33. D. R. Lide, Handbook of Chemistry and Physics, 82nd ed. (CRC Press, 2001).
  34. F. van der Heijden, Image Based Measurement System (John Wiley & Sons, 1994).
  35. T. Bose, Digital Signal and Image Processing (Wiley, 2004).
  36. A. R. Smith, “Color gamut transform pairs,” Comput. Graph. 12(3), 12–19 (1978). [CrossRef]
  37. C. L. Wong, H. P. Ho, K. S. Chan, S. Y. Wu, and C. Lin, “Application of spectral surface plasmon resonance to gas pressure sensing,” Opt. Eng. 44(12), 124403 (2005). [CrossRef]
  38. K. H. Lee, Y. D. Su, S. J. Chen, F. G. Tseng, and G. B. Lee, “Microfluidic systems integrated with two-dimensional surface plasmon resonance phase imaging systems for microarray immunoassay,” Biosens. Bioelectron. 23(4), 466–472 (2007). [CrossRef] [PubMed]
  39. P. I. Nikitin, A. N. Grigorenko, A. A. Beloglazov, M. V. Valeiko, A. I. Savchuk, O. A. Savchuk, G. Steiner, C. Kuhne, A. Huebner, and R. Salzer, “Surface plasmon resonance interferometry for micro-array biosensing,” Sens. Actuators A Phys. 85(1–3), 189–193 (2000). [CrossRef]
  40. H. P. Ho and W. W. Lam, “Application of differential phase measurement technique to surface plasmon resonance sensors,” Sens. Actuators B Chem. 96(3), 554–559 (2003). [CrossRef]
  41. X. Yu, D. Wang, X. Wei, X. Ding, W. Liao, and Z. Xinsheng, “A surface plasmon resonance imaging interferometry for protein micro-array detection,” Sens. Actuators B Chem. 108(1–2), 765–771 (2005). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited