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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 19, Iss. 20 — Sep. 26, 2011
  • pp: 19156–19168

Localization-based super-resolution microscopy with an sCMOS camera

Zhen-Li Huang, Hongyu Zhu, Fan Long, Hongqiang Ma, Lingsong Qin, Yongfeng Liu, Jiuping Ding, Zhihong Zhang, Qingming Luo, and Shaoqun Zeng  »View Author Affiliations


Optics Express, Vol. 19, Issue 20, pp. 19156-19168 (2011)
http://dx.doi.org/10.1364/OE.19.019156


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Abstract

In the community of localization-based super-resolution microscopy (or called localization microscopy), it is generally believed that the emission of single molecules is so weak that an EMCCD (electron multiplying charge coupled device) camera is necessary to be used as the detector by eliminating read noise. Here we evaluate the possibility of a new kind of low light detector, scientific complementary metal-oxide-semiconductor (sCMOS) camera in localization microscopy. We demonstrate experimentally that sCMOS is capable of imaging actin bundles with FWHM diameter of 37 nm, evidencing the capability of sCMOS in localization microscopy. We further characterize the noise performance of sCMOS and find out that, with the use of a bright fluorescence probe such as d2EosFP, localization microscopy imaging is now working in the shot noise limited region.

© 2011 OSA

OCIS Codes
(040.3780) Detectors : Low light level
(100.6640) Image processing : Superresolution
(180.2520) Microscopy : Fluorescence microscopy

ToC Category:
Microscopy

History
Original Manuscript: July 22, 2011
Revised Manuscript: August 31, 2011
Manuscript Accepted: September 5, 2011
Published: September 19, 2011

Virtual Issues
Vol. 6, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Zhen-Li Huang, Hongyu Zhu, Fan Long, Hongqiang Ma, Lingsong Qin, Yongfeng Liu, Jiuping Ding, Zhihong Zhang, Qingming Luo, and Shaoqun Zeng, "Localization-based super-resolution microscopy with an sCMOS camera," Opt. Express 19, 19156-19168 (2011)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-19-20-19156


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