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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 19, Iss. 20 — Sep. 26, 2011
  • pp: 19182–19189

Image contrast immersion method for measuring refractive index applied to spider silks

Douglas J. Little and D. M. Kane  »View Author Affiliations


Optics Express, Vol. 19, Issue 20, pp. 19182-19189 (2011)
http://dx.doi.org/10.1364/OE.19.019182


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Abstract

A technique for measuring the refractive index of micron sized fibers using a series of immersion index matching oils, and image contrast measurements is proposed and demonstrated. It has been applied to radial silks of the orb web weaving spider Plebs eburnus. These have widths of ~1-2 microns. Values about 1.5500 are obtained, with birefringence values between 0.0000 and 0.0133 for individual silks. An uncertainty in the range ± 5 × 10−4 to ± 2 × 10−3 is achieved for these challenging samples. This accuracy is about a twenty times improvement on previously reported measurements for spider silks using other techniques. The technique is used to obtain measurements of the refractive index of spider silks as a function of wavelength, for the first time. An Abbe number for the radial silks of Plebs eburnus of ~32 is found.

© 2011 OSA

OCIS Codes
(120.5710) Instrumentation, measurement, and metrology : Refraction
(260.1440) Physical optics : Birefringence
(290.3030) Scattering : Index measurements
(160.1435) Materials : Biomaterials

ToC Category:
Instrumentation, Measurement, and Metrology

History
Original Manuscript: July 14, 2011
Revised Manuscript: August 25, 2011
Manuscript Accepted: September 2, 2011
Published: September 19, 2011

Citation
Douglas J. Little and D. M. Kane, "Image contrast immersion method for measuring refractive index applied to spider silks," Opt. Express 19, 19182-19189 (2011)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-19-20-19182


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References

  1. M. F. Ashby, L. J. Gibson, U. Wegst, and R. Olive, “The mechanical properties of natural materials. I. Material property charts,” Proc. R. Soc. Lond. A450(1938), 123–140 (1995). [CrossRef]
  2. M. Heim, D. Keerl, and T. Scheibel, “Spider silk: from soluble protein to extraordinary fibre,” Angew. Chem. Int. Ed.48(20), 3584–3596 (2009). [CrossRef]
  3. J. A. Kluge, O. Rabotyagova, G. G. Leisk, and D. L. Kaplan, “Spider silks and their applications,” Trends Biotechnol.26(5), 244–251 (2008). [CrossRef] [PubMed]
  4. C. L. Craig, G. D. Bernard, and J. A. Coddington, “Evolutionary shifts in the spectral properties of spider silks,” Evolution48(2), 287–296 (1994). [CrossRef]
  5. D. M. Kane, A. M. Joyce, G. R. Staib, and M. E. Herberstein, “Optical surface profiling of orb-web spider capture silks,” Bioinspir. Biomim.5(3), 036004 (2010). [CrossRef] [PubMed]
  6. D. M. Kane, N. Naidoo, and G. R. Staib, “AFM of orb-spider-web-silks to measure surface nanostructuring and evaluate silk fibers per strand,” J. Appl. Phys.108(073509), 5 (2010). [CrossRef]
  7. D. M. Kane, G. R. Staib, N. Naidoo, D. J. Little, and M. E. Herberstein, ““Optics of spider “sticky” orb webs,” Proc. SPIE7975, 79750G, 79750G-13 (2011), doi:. [CrossRef]
  8. A. J. Werner, “Methods in high precision refractometry of optical glasses,” Appl. Opt.7(5), 837–843 (1968). [CrossRef] [PubMed]
  9. Y. S. Liu, “Direct measurement of the refractive indices for a small numerical aperture cladded fiber: a simple method,” Appl. Opt.13(6), 1255–1256 (1974). [CrossRef] [PubMed]
  10. F. Rinne and M. Berek, Anleitung zu optischen untersuchungen mit dem polarisationsmikroskop (Jänecke, Leipzig, 1935).
  11. R. G. Greenler and J. W. Hable, “Colors in spiders webs,” Am. Sci.77, 368–373 (1989).
  12. R. G. Greenler, J. W. Hable, and P. O. Slane, “Diffraction around a fine wire: how good is the single-slit approximation?” Am. J. Phys.58(4), 330–331 (1990). [CrossRef]
  13. F. Tajima and Y. Nishiyama, “Light scattering from a birefringent cylinder, spider silk, slimmer than the wavelength approaches dipole radiation,” J. Opt. Soc. Am. A22(6), 1127–1131 (2005). [CrossRef] [PubMed]
  14. F. Tajima and Y. Nishiyama, “Multiple scattering effect in the Young-like interference pattern of an optical wave scattered by a double cylinder,” Opt. Rev.15(2), 75–83 (2008). [CrossRef]
  15. R. C. Emmons and R. M. Gates, “The use of Becke line colors in refractive index determination,” Am. Mineral.33, 612–618 (1948).
  16. S. Carmichael and C. Viney, “Molecular order in spider major ampullate silk (dragline): effects of spinning rate and post-spin drawing,” J. Appl. Polym. Sci.72(7), 895–903 (1999). [CrossRef]
  17. H.-G. Zimmer, Geometrische Optik (Springer, 1967), p. 80.
  18. N. Naidoo, D. Birch, M. E. Herberstein and D. M. Kane, “Nano-morphology and radial cross-section characterisation of dragline/structural silks of Araneidae spiders,” Bioinspir. Biomim. (submitted for publication).

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