OSA's Digital Library

Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 19, Iss. 20 — Sep. 26, 2011
  • pp: 19653–19659

Dual-band spectral-domain optical coherence tomography for in vivo imaging the spectral contrasts of the retinal nerve fiber layer

Xiangyang Zhang, Jianming Hu, Robert W. Knighton, Xiang-Run Huang, Carmen A. Puliafito, and Shuliang Jiao  »View Author Affiliations


Optics Express, Vol. 19, Issue 20, pp. 19653-19659 (2011)
http://dx.doi.org/10.1364/OE.19.019653


View Full Text Article

Enhanced HTML    Acrobat PDF (1171 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

The ultimate goal of the study is to provide an imaging tool to detect the earliest signs of glaucoma before clinically visible damage occurs to the retinal nerve fiber layer (RNFL). Studies have shown that the optical reflectance of the damaged RNFL at short wavelength (<560nm) is reduced much more than that at long wavelength, which provides spectral contrast for imaging the earliest damage to the RNFL. To image the spectral contrast we built a dual-band spectral-domain optical coherence tomography (SD-OCT) centered at 808nm (NIR) and 415nm (VIS). The light at the two bands was provided by the fundamental and frequency-doubled outputs of a broadband Ti:Sapphire laser. The depth resolution of the NIR and VIS OCT systems are 4.7µm and 12.2µm in the air, respectively. The system was applied to imaging the rat retina in vivo. Significantly different appearances between the OCT cross sectional images at the two bands were observed. The ratio of the light reflected from the RNFL over that reflected from the entire retina at the two bands were quantitatively compared. The experimental results showed that the dual-band OCT system is feasible for imaging the spectral contrasts of the RNFL.

© 2011 OSA

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.1610) Medical optics and biotechnology : Clinical applications
(170.4460) Medical optics and biotechnology : Ophthalmic optics and devices
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: August 8, 2011
Revised Manuscript: September 7, 2011
Manuscript Accepted: September 10, 2011
Published: September 22, 2011

Virtual Issues
Vol. 6, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Xiangyang Zhang, Jianming Hu, Robert W. Knighton, Xiang-Run Huang, Carmen A. Puliafito, and Shuliang Jiao, "Dual-band spectral-domain optical coherence tomography for in vivo imaging the spectral contrasts of the retinal nerve fiber layer," Opt. Express 19, 19653-19659 (2011)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-19-20-19653


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. H. A. Quigley, “Glaucoma,” Lancet377(9774), 1367–1377 (2011). [CrossRef] [PubMed]
  2. L. A. Kerrigan-Baumrind, H. A. Quigley, M. E. Pease, D. F. Kerrigan, and R. S. Mitchell, “Number of ganglion cells in glaucoma eyes compared with threshold visual field tests in the same persons,” Invest. Ophthalmol. Vis. Sci.41(3), 741–748 (2000). [PubMed]
  3. M. L. Gabriele, G. Wollstein, H. Ishikawa, L. Kagemann, J. Xu, L. S. Folio, and J. S. Schuman, “Optical coherence tomography: history, current status, and laboratory work,” Invest. Ophthalmol. Vis. Sci.52(5), 2425–2436 (2011). [CrossRef] [PubMed]
  4. H. G. Lemij and N. J. Reus, “New developments in scanning laser polarimetry for glaucoma,” Curr. Opin. Ophthalmol.19(2), 136–140 (2008). [CrossRef] [PubMed]
  5. R. W. Knighton, X. Huang, and Q. Zhou, “Microtubule contribution to the reflectance of the retinal nerve fiber layer,” Invest. Ophthalmol. Vis. Sci.39(1), 189–193 (1998). [PubMed]
  6. X.-R. Huang and R. W. Knighton, “Microtubules contribute to the birefringence of the retinal nerve fiber layer,” Invest. Ophthalmol. Vis. Sci.46(12), 4588–4593 (2005). [CrossRef] [PubMed]
  7. B. Fortune, L. Wang, G. Cull, and G. A. Cioffi, “Intravitreal colchicine causes decreased RNFL birefringence without altering RNFL thickness,” Invest. Ophthalmol. Vis. Sci.49(1), 255–261 (2008). [CrossRef] [PubMed]
  8. G. M. Pocock, R. G. Aranibar, N. J. Kemp, C. S. Specht, M. K. Markey, and H. G. Rylander, “The relationship between retinal ganglion cell axon constituents and retinal nerve fiber layer birefringence in the primate,” Invest. Ophthalmol. Vis. Sci.50(11), 5238–5246 (2009). [CrossRef] [PubMed]
  9. X.-R. Huang and R. W. Knighton, “Altered F-actin distribution in retinal nerve fiber layer of a rat model of glaucoma,” Exp. Eye Res.88(6), 1107–1114 (2009). [CrossRef] [PubMed]
  10. X.-R. Huang, W. Kong, Y. Zhou, and G. Gregori, “Distortion of axonal cytoskeleton: an early sign of glaucomatous damage,” Invest. Ophthalmol. Vis. Sci.52(6), 2879–2888 (2011). [CrossRef] [PubMed]
  11. X.-R. Huang, Y. Zhou, W. Kong, and R. W. Knighton, “Reflectance decrease prior to thickness change of the retinal nerve fiber layer in glaucomatous retinas”, Invest. Ophthalmol. Vis. Sci., published ahead of print July 5, 2011.
  12. R. W. Knighton and X.-R. Huang, “Directional and spectral reflectance of the rat retinal nerve fiber layer,” Invest. Ophthalmol. Vis. Sci.40(3), 639–647 (1999). [PubMed]
  13. X.-R. Huang, Y. Zhou, W. Kong, and R. W. Knighton, “Change of retinal nerve fiber layer reflectance correlated with cytostructural change in glaucoma,” ARVO Meeting Abstracts (Fort Lauderdale, Florida 2011), 52:2442.
  14. M. Ruggeri, G. Tsechpenakis, S. Jiao, M. E. Jockovich, C. Cebulla, E. Hernandez, T. G. Murray, and C. A. Puliafito, “Retinal tumor imaging and volume quantification in mouse model using spectral-domain optical coherence tomography,” Opt. Express17(5), 4074–4083 (2009). [CrossRef] [PubMed]
  15. X. Zhang, H. F. Zhang, C. A. Puliafito, and S. Jiao, “Simultaneous in vivo imaging of melanin and lipofuscin in the retina with photoacoustic ophthalmoscopy and autofluorescence imaging,” J. Biomed. Opt.16(8), 080504 (2011). [CrossRef] [PubMed]
  16. R. W. Knighton and X.-R. Huang, “Visible and near-infrared imaging of the nerve fiber layer of the isolated rat retina,” J. Glaucoma8(1), 31–37 (1999). [CrossRef] [PubMed]
  17. Scott Prahl, “Optical Absorption of Hemoglobin,” http://omlc.ogi.edu/spectra/hemoglobin/summary.html .

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited