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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 19, Iss. 21 — Oct. 10, 2011
  • pp: 20571–20579

Dispersion-relation phase spectroscopy of intracellular transport

Ru Wang, Zhuo Wang, Larry Millet, Martha U. Gillette, A. J. Levine, and Gabriel Popescu  »View Author Affiliations


Optics Express, Vol. 19, Issue 21, pp. 20571-20579 (2011)
http://dx.doi.org/10.1364/OE.19.020571


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Abstract

We used quantitative phase imaging to measure the dispersion relation, i.e. decay rate vs. spatial mode, associated with mass transport in live cells. This approach applies equally well to both discrete and continuous mass distributions without the need for particle tracking. From the quadratic experimental curve specific to diffusion, we extracted the diffusion coefficient as the only fitting parameter. The linear portion of the dispersion relation reveals the deterministic component of the intracellular transport. Our data show a universal behavior where the intracellular transport is diffusive at small scales and deterministic at large scales. Measurements by our method and particle tracking show that, on average, the mass transport in the nucleus is slower than in the cytoplasm.

© 2011 OSA

OCIS Codes
(000.2700) General : General science
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(180.0180) Microscopy : Microscopy

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: August 3, 2011
Revised Manuscript: September 12, 2011
Manuscript Accepted: September 18, 2011
Published: October 3, 2011

Virtual Issues
Vol. 6, Iss. 11 Virtual Journal for Biomedical Optics

Citation
Ru Wang, Zhuo Wang, Larry Millet, Martha U. Gillette, A. J. Levine, and Gabriel Popescu, "Dispersion-relation phase spectroscopy of intracellular transport," Opt. Express 19, 20571-20579 (2011)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-19-21-20571


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