OSA's Digital Library

Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 19, Iss. 24 — Nov. 21, 2011
  • pp: 23716–23726

Parallelized STED fluorescence nanoscopy

Pit Bingen, Matthias Reuss, Johann Engelhardt, and Stefan W. Hell  »View Author Affiliations


Optics Express, Vol. 19, Issue 24, pp. 23716-23726 (2011)
http://dx.doi.org/10.1364/OE.19.023716


View Full Text Article

Enhanced HTML    Acrobat PDF (4936 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We introduce a parallelized STED microscope featuring m = 4 pairs of scanning excitation and STED beams, providing m-fold increased imaging speed of a given sample area, while maintaining basically all of the advantages of single beam scanning. Requiring only a single laser source and fiber input, the setup is inherently aligned both spatially and temporally. Given enough laser power, the design is readily scalable to higher degrees of parallelization m.

© 2011 OSA

OCIS Codes
(000.2170) General : Equipment and techniques
(180.2520) Microscopy : Fluorescence microscopy
(350.5730) Other areas of optics : Resolution
(080.4865) Geometric optics : Optical vortices

ToC Category:
Microscopy

History
Original Manuscript: September 13, 2011
Revised Manuscript: October 29, 2011
Manuscript Accepted: October 31, 2011
Published: November 7, 2011

Virtual Issues
Vol. 7, Iss. 1 Virtual Journal for Biomedical Optics

Citation
Pit Bingen, Matthias Reuss, Johann Engelhardt, and Stefan W. Hell, "Parallelized STED fluorescence nanoscopy," Opt. Express 19, 23716-23726 (2011)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-19-24-23716


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. S. W. Hell and J. Wichmann, “Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy,” Opt. Lett.19(11), 780–782 (1994). [CrossRef] [PubMed]
  2. T. A. Klar and S. W. Hell, “Subdiffraction resolution in far-field fluorescence microscopy,” Opt. Lett.24(14), 954–956 (1999). [CrossRef] [PubMed]
  3. S. W. Hell and M. Kroug, “Ground-state depletion fluorescence microscopy, a concept for breaking the diffraction resolution limit,” Appl. Phys. B60(5), 495–497 (1995). [CrossRef]
  4. R. Heintzmann, T. M. Jovin, and C. Cremer, “Saturated patterned excitation microscopy--a concept for optical resolution improvement,” J. Opt. Soc. Am. A19(8), 1599–1609 (2002). [CrossRef] [PubMed]
  5. S. W. Hell, S. Jakobs, and L. Kastrup, “Imaging and writing at the nanoscale with focused visible light through saturable optical transitions,” Appl. Phys., A Mater. Sci. Process.77(7), 859–860 (2003). [CrossRef]
  6. E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science313(5793), 1642–1645 (2006). [CrossRef] [PubMed]
  7. M. J. Rust, M. Bates, and X. W. Zhuang, “Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM),” Nat. Methods3(10), 793–796 (2006). [CrossRef] [PubMed]
  8. S. T. Hess, T. P. K. Girirajan, and M. D. Mason, “Ultra-high resolution imaging by fluorescence photoactivation localization microscopy,” Biophys. J.91(11), 4258–4272 (2006). [CrossRef] [PubMed]
  9. S. W. Hell, “Far-field optical nanoscopy,” Science316(5828), 1153–1158 (2007). [CrossRef] [PubMed]
  10. S. W. Hell, “Microscopy and its focal switch,” Nat. Methods6(1), 24–32 (2009). [CrossRef] [PubMed]
  11. B. Huang, H. Babcock, and X. Zhuang, “Breaking the diffraction barrier: super-resolution imaging of cells,” Cell143(7), 1047–1058 (2010). [CrossRef] [PubMed]
  12. T. A. Klar, S. Jakobs, M. Dyba, A. Egner, and S. W. Hell, “Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission,” Proc. Natl. Acad. Sci. U.S.A.97(15), 8206–8210 (2000). [CrossRef] [PubMed]
  13. T. A. Klar, E. Engel, and S. W. Hell, “Breaking Abbe’s diffraction resolution limit in fluorescence microscopy with stimulated emission depletion beams of various shapes,” Phys. Rev. E Stat. Nonlin. Soft Matter Phys.64(6), 066613 (2001). [CrossRef] [PubMed]
  14. S. W. Hell, “Toward fluorescence nanoscopy,” Nat. Biotechnol.21(11), 1347–1355 (2003). [CrossRef] [PubMed]
  15. S. W. Hell, “Increasing the resolution of far-field fluorescence light microscopy by point-spread-function engineering,” in Topics in Fluorescence Spectroscopy, J. R. Lakowicz, ed. (Plenum Press, New York, 1997), pp. 361–422.
  16. A. Ichihara, T. Tanaami, K. Isozaki, Y. Sugiyama, Y. Kosugi, K. Mikuriya, M. Abe, and I. Uemura, “High-speed confocal fluorescence microscopy using a Nipkow scanner with microlenses for 3D-imaging of single fluorescent molecule in real time,” Bioimaging4, 52–62 (1996).
  17. M. G. L. Gustafsson, “Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy,” J. Microsc.198(2), 82–87 (2000). [CrossRef] [PubMed]
  18. M. G. L. Gustafsson, “Nonlinear structured-illumination microscopy: wide-field fluorescence imaging with theoretically unlimited resolution,” Proc. Natl. Acad. Sci. U.S.A.102(37), 13081–13086 (2005). [CrossRef] [PubMed]
  19. J. Keller, A. Schönle, and S. W. Hell, “Efficient fluorescence inhibition patterns for RESOLFT microscopy,” Opt. Express15(6), 3361–3371 (2007). [CrossRef] [PubMed]
  20. M. A. Schwentker, H. Bock, M. Hofmann, S. Jakobs, J. Bewersdorf, C. Eggeling, and S. W. Hell, “Wide-field subdiffraction RESOLFT microscopy using fluorescent protein photoswitching,” Microsc. Res. Tech.70(3), 269–280 (2007). [CrossRef] [PubMed]
  21. M. Reuss, J. Engelhardt, and S. W. Hell, “Birefringent device converts a standard scanning microscope into a STED microscope that also maps molecular orientation,” Opt. Express18(2), 1049–1058 (2010). [CrossRef] [PubMed]
  22. G. Wong, R. Pilkington, and A. R. Harvey, “Achromatization of Wollaston polarizing beam splitters,” Opt. Lett.36(8), 1332–1334 (2011). [CrossRef] [PubMed]
  23. D. Wildanger, J. Bückers, V. Westphal, S. W. Hell, and L. Kastrup, “A STED microscope aligned by design,” Opt. Express17(18), 16100–16110 (2009). [CrossRef] [PubMed]
  24. K. I. Willig, S. O. Rizzoli, V. Westphal, R. Jahn, and S. W. Hell, “STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis,” Nature440(7086), 935–939 (2006). [CrossRef] [PubMed]
  25. G. Donnert, C. Eggeling, and S. W. Hell, “Major signal increase in fluorescence microscopy through dark-state relaxation,” Nat. Methods4(1), 81–86 (2007). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited