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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 19, Iss. 24 — Nov. 21, 2011
  • pp: 23831–23844

In vivo depth-resolved oxygen saturation by dual-wavelength photothermal (DWP) OCT

Roman V. Kuranov, Shams Kazmi, Austin B. McElroy, Jeffrey W. Kiel, Andrew K. Dunn, Thomas E. Milner, and Timothy Q. Duong  »View Author Affiliations


Optics Express, Vol. 19, Issue 24, pp. 23831-23844 (2011)
http://dx.doi.org/10.1364/OE.19.023831


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Abstract

Microvasculature hemoglobin oxygen saturation (SaO2) is important in the progression of various pathologies. Non-invasive depth-resolved measurement of SaO2 levels in tissue microvasculature has the potential to provide early biomarkers and a better understanding of the pathophysiological processes allowing improved diagnostics and prediction of disease progression. We report proof-of-concept in vivo depth-resolved measurement of SaO2 levels in selected 30 µm diameter arterioles in the murine brain using Dual-Wavelength Photothermal (DWP) Optical Coherence Tomography (OCT) with 800 nm and 770 nm photothermal excitation wavelengths. Depth location of back-reflected light from a target arteriole was confirmed using Doppler and speckle contrast OCT images. SaO2 measured in a murine arteriole with DWP-OCT is linearly correlated (R2=0.98) with systemic SaO2 values recorded by a pulse-oximeter. DWP-OCT are steadily lower (10.1%) than systemic SaO2 values except during pure oxygen breathing. DWP-OCT is insensitive to OCT intensity variations and is a candidate approach for in vivo depth-resolved quantitative imaging of microvascular SaO2 levels.

© 2011 OSA

OCIS Codes
(120.5050) Instrumentation, measurement, and metrology : Phase measurement
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(300.1030) Spectroscopy : Absorption

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: July 21, 2011
Revised Manuscript: September 19, 2011
Manuscript Accepted: September 19, 2011
Published: November 9, 2011

Virtual Issues
Vol. 7, Iss. 1 Virtual Journal for Biomedical Optics

Citation
Roman V. Kuranov, Shams Kazmi, Austin B. McElroy, Jeffrey W. Kiel, Andrew K. Dunn, Thomas E. Milner, and Timothy Q. Duong, "In vivo depth-resolved oxygen saturation by dual-wavelength photothermal (DWP) OCT," Opt. Express 19, 23831-23844 (2011)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-19-24-23831


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