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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 19, Iss. 24 — Nov. 21, 2011
  • pp: 24589–24595

Holographic aberration correction: optimising the stiffness of an optical trap deep in the sample

Maria Dienerowitz, Graham Gibson, Richard Bowman, and Miles Padgett  »View Author Affiliations

Optics Express, Vol. 19, Issue 24, pp. 24589-24595 (2011)

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We investigate the effects of 1st order spherical aberration and defocus upon the stiffness of an optical trap tens of μm into the sample. We control both these aberrations with a spatial light modulator. The key to maintain optimum trap stiffness over a range of depths is a specific non-trivial combination of defocus and axial objective position. This optimisation increases the trap stiffness by up to a factor of 3 and allows trapping of 1μm polystyrene beads up to 50μm deep in the sample.

© 2011 OSA

OCIS Codes
(090.1000) Holography : Aberration compensation
(140.7010) Lasers and laser optics : Laser trapping
(230.6120) Optical devices : Spatial light modulators
(350.4855) Other areas of optics : Optical tweezers or optical manipulation

ToC Category:
Optical Trapping and Manipulation

Original Manuscript: August 15, 2011
Revised Manuscript: October 25, 2011
Manuscript Accepted: October 27, 2011
Published: November 16, 2011

Virtual Issues
Vol. 7, Iss. 1 Virtual Journal for Biomedical Optics

Maria Dienerowitz, Graham Gibson, Richard Bowman, and Miles Padgett, "Holographic aberration correction: optimising the stiffness of an optical trap deep in the sample," Opt. Express 19, 24589-24595 (2011)

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