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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 19, Iss. 27 — Dec. 19, 2011
  • pp: 26044–26055

Dark-field digital holographic microscopy for 3D-tracking of gold nanoparticles

F. Verpillat, F. Joud, P. Desbiolles, and M. Gross  »View Author Affiliations

Optics Express, Vol. 19, Issue 27, pp. 26044-26055 (2011)

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We present a new technique that combines off-axis Digital Holography and Dark Field Microscopy to track 100nm gold particles diffusing in water. We show that a single hologram is sufficient to localize several particles in a thick sample with a localization accuracy independent of the particle position. From our measurements we reconstruct the trajectories of the particles and derive their 3D diffusion coefficient. Our results pave the way for quantitative studies of the motion of single nanoparticle in complex media.

© 2011 OSA

OCIS Codes
(090.1760) Holography : Computer holography
(180.0180) Microscopy : Microscopy
(290.5850) Scattering : Scattering, particles
(090.1995) Holography : Digital holography
(100.4999) Image processing : Pattern recognition, target tracking

ToC Category:

Original Manuscript: September 14, 2011
Revised Manuscript: November 9, 2011
Manuscript Accepted: November 13, 2011
Published: December 7, 2011

Virtual Issues
Vol. 7, Iss. 2 Virtual Journal for Biomedical Optics

F. Verpillat, F. Joud, P. Desbiolles, and M. Gross, "Dark-field digital holographic microscopy for 3D-tracking of gold nanoparticles," Opt. Express 19, 26044-26055 (2011)

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