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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 19, Iss. 4 — Feb. 14, 2011
  • pp: 3130–3143

Simultaneous multi-lifetime multi-color STED imaging for colocalization analyses

Johanna Bückers, Dominik Wildanger, Giuseppe Vicidomini, Lars Kastrup, and Stefan W. Hell  »View Author Affiliations

Optics Express, Vol. 19, Issue 4, pp. 3130-3143 (2011)

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We describe a STED microscope optimized for colocalization experiments with up to three colors. Two fluorescence labels are separated by their fluorescence lifetime whereas a third channel is discriminated by the wavelength of fluorescence emission. Since it does not require a second STED beam, separating by lifetime is insensitive to drift and thus optimally suited for colocalization analyses. Furthermore, we propose a setup having a second STED beam for long duration multicolor recording.

© 2011 OSA

OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(350.5730) Other areas of optics : Resolution

ToC Category:

Original Manuscript: January 4, 2011
Manuscript Accepted: January 18, 2011
Published: February 2, 2011

Virtual Issues
Vol. 6, Iss. 3 Virtual Journal for Biomedical Optics

Johanna Bückers, Dominik Wildanger, Giuseppe Vicidomini, Lars Kastrup, and Stefan W. Hell, "Simultaneous multi-lifetime multi-color STED imaging for colocalization analyses," Opt. Express 19, 3130-3143 (2011)

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