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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 19, Iss. 4 — Feb. 14, 2011
  • pp: 3175–3184

Blind source unmixing in multi-spectral optoacoustic tomography

Jürgen Glatz, Nikolaos C. Deliolanis, Andreas Buehler, Daniel Razansky, and Vasilis Ntziachristos  »View Author Affiliations


Optics Express, Vol. 19, Issue 4, pp. 3175-3184 (2011)
http://dx.doi.org/10.1364/OE.19.003175


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Abstract

Multispectral optoacoustic (photoacoustic) tomography (MSOT) is a hybrid modality that can image through several millimeters to centimeters of diffuse tissues, attaining resolutions typical of ultrasound imaging. The method can further identify tissue biomarkers by decomposing the spectral contributions of different photo-absorbing molecules of interest. In this work we investigate the performance of blind source unmixing methods and spectral fitting approaches in decomposing the contributions of fluorescent dyes from the tissue background, based on MSOT measurements in mice. We find blind unmixing as a promising method for accurate MSOT decomposition, suitable also for spectral unmixing in fluorescence imaging. We further demonstrate its capacity with temporal unmixing on real-time MSOT data obtained in-vivo for enhancing the visualization of absorber agent flow in the mouse vascular system.

© 2011 Optical Society of America

OCIS Codes
(170.5120) Medical optics and biotechnology : Photoacoustic imaging
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(170.6960) Medical optics and biotechnology : Tomography
(100.1455) Image processing : Blind deconvolution

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: November 2, 2010
Revised Manuscript: January 25, 2011
Manuscript Accepted: January 30, 2011
Published: February 3, 2011

Virtual Issues
Vol. 6, Iss. 3 Virtual Journal for Biomedical Optics

Citation
Jürgen Glatz, Nikolaos C. Deliolanis, Andreas Buehler, Daniel Razansky, and Vasilis Ntziachristos, "Blind source unmixing in multi-spectral optoacoustic tomography," Opt. Express 19, 3175-3184 (2011)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-19-4-3175


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