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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 19, Iss. 5 — Feb. 28, 2011
  • pp: 3862–3869

Coherent noise reduction in digital holographic phase contrast microscopy by slightly shifting object

Feng Pan, Wen Xiao, Shuo Liu, FanJing Wang, Lu Rong, and Rui Li  »View Author Affiliations

Optics Express, Vol. 19, Issue 5, pp. 3862-3869 (2011)

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A method to reduce coherent noise in digital holographic phase contrast microscopy is proposed. By slightly shifting the specimen, a series of digital holograms with different coherent noise patterns is recorded. Each hologram is reconstructed individually, while the different phase tilts of the reconstructed complex amplitudes due to the specimen shifts are corrected in the hologram plane by using numerical parametric lens method. Afterward, the lateral displacements of the phase maps from different holograms are compensated in the image plane by using digital image registration method. Thus, all phase images have same distribution, but uncorrelated coherent noise patterns. By a proper averaging procedure, the coherent noise of phase contrast image is reduced significantly. The experimental results are given to confirm the proposed method.

© 2011 OSA

OCIS Codes
(100.2980) Image processing : Image enhancement
(100.3010) Image processing : Image reconstruction techniques
(180.3170) Microscopy : Interference microscopy
(090.1995) Holography : Digital holography

ToC Category:

Original Manuscript: November 16, 2010
Revised Manuscript: January 4, 2011
Manuscript Accepted: February 3, 2011
Published: February 14, 2011

Virtual Issues
Vol. 6, Iss. 3 Virtual Journal for Biomedical Optics

Feng Pan, Wen Xiao, Shuo Liu, FanJing Wang, Lu Rong, and Rui Li, "Coherent noise reduction in digital holographic phase contrast microscopy by slightly shifting object," Opt. Express 19, 3862-3869 (2011)

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