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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 19, Iss. 6 — Mar. 14, 2011
  • pp: 4833–4847

Brain refractive index measured in vivo with high-NA defocus-corrected full-field OCT and consequences for two-photon microscopy

Jonas Binding, Juliette Ben Arous, Jean-François Léger, Sylvain Gigan, Claude Boccara, and Laurent Bourdieu  »View Author Affiliations

Optics Express, Vol. 19, Issue 6, pp. 4833-4847 (2011)

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Two-photon laser scanning microscopy (2PLSM) is an important tool for in vivo tissue imaging with sub-cellular resolution, but the penetration depth of current systems is potentially limited by sample-induced optical aberrations. To quantify these, we measured the refractive index n' in the somatosensory cortex of 7 rats in vivo using defocus optimization in full-field optical coherence tomography (ff-OCT). We found n' to be independent of imaging depth or rat age. From these measurements, we calculated that two-photon imaging beyond 200µm into the cortex is limited by spherical aberration, indicating that adaptive optics will improve imaging depth.

© 2011 OSA

OCIS Codes
(080.1010) Geometric optics : Aberrations (global)
(110.4500) Imaging systems : Optical coherence tomography
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(290.0290) Scattering : Scattering
(290.3030) Scattering : Index measurements
(110.0113) Imaging systems : Imaging through turbid media
(180.4315) Microscopy : Nonlinear microscopy
(170.6935) Medical optics and biotechnology : Tissue characterization
(110.1080) Imaging systems : Active or adaptive optics

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: December 7, 2010
Revised Manuscript: January 24, 2011
Manuscript Accepted: February 2, 2011
Published: February 28, 2011

Virtual Issues
Vol. 6, Iss. 4 Virtual Journal for Biomedical Optics

Jonas Binding, Juliette Ben Arous, Jean-François Léger, Sylvain Gigan, Claude Boccara, and Laurent Bourdieu, "Brain refractive index measured in vivo with high-NA defocus-corrected full-field OCT and consequences for two-photon microscopy," Opt. Express 19, 4833-4847 (2011)

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