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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 19, Iss. 6 — Mar. 14, 2011
  • pp: 5536–5550

Label-free imaging of intracellular motility by low-coherent quantitative phase microscopy

Toyohiko Yamauchi, Hidenao Iwai, and Yutaka Yamashita  »View Author Affiliations

Optics Express, Vol. 19, Issue 6, pp. 5536-5550 (2011)

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The subject study demonstrates the imaging of cell activity by quantitatively assessing the motion of intracellular organelles and cell plasma membranes without any contrast agent. The low-coherent interferometric technique and phase-referenced phase shifting technique were integrated to reveal the depth-resolved distribution of intracellular motility. The transversal and vertical spatial resolutions were 0.56 μm and 0.93 μm, respectively, and the mechanical stability of the system was 1.2 nm. The motility of the cell was assessed by mean squared displacement (MSD) and we have compensated for the MSD by applying statistical noise analysis. Thus we show the significant change of intracellular motility after paraformaldehyde treatment in non-labeled cells.

© 2011 OSA

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(180.3170) Microscopy : Interference microscopy
(240.6648) Optics at surfaces : Surface dynamics

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: January 31, 2011
Revised Manuscript: February 28, 2011
Manuscript Accepted: February 28, 2011
Published: March 9, 2011

Virtual Issues
Vol. 6, Iss. 4 Virtual Journal for Biomedical Optics

Toyohiko Yamauchi, Hidenao Iwai, and Yutaka Yamashita, "Label-free imaging of intracellular motility by low-coherent quantitative phase microscopy," Opt. Express 19, 5536-5550 (2011)

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