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Optics Express

Optics Express

  • Editor: C. Martijin de Sterke
  • Vol. 19, Iss. 7 — Mar. 28, 2011
  • pp: 6571–6586

Amplitude and phase images of cellular structures with a scanning surface plasmon microscope

L. Berguiga, T. Roland, K. Monier, J. Elezgaray, and F. Argoul  »View Author Affiliations


Optics Express, Vol. 19, Issue 7, pp. 6571-6586 (2011)
http://dx.doi.org/10.1364/OE.19.006571


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Abstract

Imaging cellular internal structure at nanometer scale axial resolution with non invasive microscopy techniques has been a major technical challenge since the nineties. We propose here a complement to fluorescence based microscopies with no need of staining the biological samples, based on a Scanning Surface Plasmon Microscope (SSPM). We describe the advantages of this microscope, namely the possibility of both amplitude and phase imaging and, due to evanescent field enhancement by the surface plasmon resonance, a very high resolution in Z scanning (Z being the axis normal to the sample). We show for fibroblast cells (IMR90) that SSPM offers an enhanced detection of index gradient regions, and we conclude it is very well suited to discriminate regions of variable density in biological media such as cell compartments, nucleus, nucleoli and membranes.

© 2011 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(170.0110) Medical optics and biotechnology : Imaging systems
(240.5420) Optics at surfaces : Polaritons
(240.6680) Optics at surfaces : Surface plasmons
(110.3175) Imaging systems : Interferometric imaging
(110.5086) Imaging systems : Phase unwrapping

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: October 27, 2010
Revised Manuscript: December 21, 2010
Manuscript Accepted: December 23, 2010
Published: March 23, 2011

Virtual Issues
Vol. 6, Iss. 4 Virtual Journal for Biomedical Optics

Citation
L. Berguiga, T. Roland, K. Monier, J. Elezgaray, and F. Argoul, "Amplitude and phase images of cellular structures with a scanning surface plasmon microscope," Opt. Express 19, 6571-6586 (2011)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-19-7-6571


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