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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 19, Iss. 8 — Apr. 11, 2011
  • pp: 7587–7595

Single-shot Full-field reflection phase microscopy

Zahid Yaqoob, Toyohiko Yamauchi, Wonshik Choi, Dan Fu, Ramachandra R. Dasari, and Michael S. Feld  »View Author Affiliations

Optics Express, Vol. 19, Issue 8, pp. 7587-7595 (2011)

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We present a full-field reflection phase microscope that combines low-coherence interferometry and off-axis digital holographic microscopy (DHM). The reflection-based DHM provides highly sensitive and a single-shot imaging of cellular dynamics while the use of low coherence source provides a depth-selective measurement. The setup uniquely uses a diffraction grating in the reference arm to generate an interference image of uniform contrast over the entire field-of-view albeit low-coherence light source. We have measured the path-length sensitivity of our instrument to be approximately 21 ‚ÄČ p i c o m e t e r s / H z that makes it suitable for nanometer-scale full-field measurement of membrane dynamics in live cells.

© 2011 OSA

OCIS Codes
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(170.1530) Medical optics and biotechnology : Cell analysis
(170.4500) Medical optics and biotechnology : Optical coherence tomography

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: February 7, 2011
Revised Manuscript: March 17, 2011
Manuscript Accepted: March 18, 2011
Published: April 5, 2011

Virtual Issues
Vol. 6, Iss. 5 Virtual Journal for Biomedical Optics

Zahid Yaqoob, Toyohiko Yamauchi, Wonshik Choi, Dan Fu, Ramachandra R. Dasari, and Michael S. Feld, "Single-shot Full-field reflection phase microscopy," Opt. Express 19, 7587-7595 (2011)

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