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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 19, Iss. 9 — Apr. 25, 2011
  • pp: 7971–7978

Detection of bacteria using bacteriophages as recognition elements immobilized on long-period fiber gratings

Mateusz Smietana, Wojtek J. Bock, Predrag Mikulic, Andy Ng, Raja Chinnappan, and Mohammed Zourob  »View Author Affiliations

Optics Express, Vol. 19, Issue 9, pp. 7971-7978 (2011)

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The paper presents for the first time a study of long-period gratings (LPGs) applied for label-free detection of specific bacteria using physically adsorbed bacteriophages. For the purposes of the experiment a number of highly sensitive LPGs working at the turning point of phase matching curve was fabricated in SMF28 fiber using UV exposure. We show that the device allows for real-time monitoring of phenomena taking place on the sensor’s surface, including phage-bacteria interactions. For the applied conditions a resonance wavelength shift of ~1.3 nm induced by bacteria binding was observed.

© 2011 OSA

OCIS Codes
(050.2770) Diffraction and gratings : Gratings
(060.2370) Fiber optics and optical communications : Fiber optics sensors
(170.1610) Medical optics and biotechnology : Clinical applications
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(280.1415) Remote sensing and sensors : Biological sensing and sensors
(280.4788) Remote sensing and sensors : Optical sensing and sensors

ToC Category:

Original Manuscript: February 17, 2011
Revised Manuscript: April 3, 2011
Manuscript Accepted: April 4, 2011
Published: April 11, 2011

Virtual Issues
Vol. 6, Iss. 5 Virtual Journal for Biomedical Optics

Mateusz Smietana, Wojtek J. Bock, Predrag Mikulic, Andy Ng, Raja Chinnappan, and Mohammed Zourob, "Detection of bacteria using bacteriophages as recognition elements immobilized on long-period fiber gratings," Opt. Express 19, 7971-7978 (2011)

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