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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 20, Iss. 10 — May. 7, 2012
  • pp: 11496–11505

Digital holographic microscopy long-term and real-time monitoring of cell division and changes under simulated zero gravity

Feng Pan, Shuo Liu, Zhe Wang, Peng Shang, and Wen Xiao  »View Author Affiliations


Optics Express, Vol. 20, Issue 10, pp. 11496-11505 (2012)
http://dx.doi.org/10.1364/OE.20.011496


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Abstract

The long-term and real-time monitoring the cell division and changes of osteoblasts under simulated zero gravity condition were succeed by combing a digital holographic microscopy (DHM) with a superconducting magnet (SM). The SM could generate different magnetic force fields in a cylindrical cavity, where the gravitational force of biological samples could be canceled at a special gravity position by a high magnetic force. Therefore the specimens were levitated and in a simulated zero gravity environment. The DHM was modified to fit with SM by using single mode optical fibers and a vertically-configured jig designed to hold specimens and integrate optical device in the magnet’s bore. The results presented the first-phase images of living cells undergoing dynamic divisions and changes under simulated zero gravity environment for a period of 10 hours. The experiments demonstrated that the SM-compatible DHM setup could provide a highly efficient and versatile method for research on the effects of microgravity on biological samples.

© 2012 OSA

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.0180) Microscopy : Microscopy
(090.1995) Holography : Digital holography

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: March 6, 2012
Revised Manuscript: April 27, 2012
Manuscript Accepted: May 1, 2012
Published: May 4, 2012

Virtual Issues
Vol. 7, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Feng Pan, Shuo Liu, Zhe Wang, Peng Shang, and Wen Xiao, "Digital holographic microscopy long-term and real-time monitoring of cell division and changes under simulated zero gravity," Opt. Express 20, 11496-11505 (2012)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-20-10-11496


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