|
The noise-limited-resolution for stimulated emission depletion microscopy of diffusing particles |
Optics Express, Vol. 20, Issue 12, pp. 12793-12798 (2012)
http://dx.doi.org/10.1364/OE.20.012793
Enhanced HTML 
Acrobat PDF (665 KB)
Abstract
With recent developments in microscopy, such as stimulated emission depletion (STED) microscopy, far-field imaging at resolutions better than the diffraction limit is now a commercially available technique. Here, we show that, in the special case of a diffusive regime, the noise-limited resolution of STED imaging is independent of the saturation intensity of the fluorescent label. Thermal motion limits the signal integration time, which, for a given excited-state lifetime, limits the total number of photons available for detection.
© 2012 OSA
OCIS Codes
(110.0110) Imaging systems : Imaging systems
(110.0180) Imaging systems : Microscopy
(110.4280) Imaging systems : Noise in imaging systems
(180.2520) Microscopy : Fluorescence microscopy
ToC Category:
Microscopy
History
Original Manuscript: February 28, 2012
Revised Manuscript: April 19, 2012
Manuscript Accepted: April 24, 2012
Published: May 23, 2012
Virtual Issues
Vol. 7, Iss. 8 Virtual Journal for Biomedical Optics
Citation
Chris J. Lee and Klaus J. Boller, "The noise-limited-resolution for stimulated emission depletion microscopy of diffusing particles," Opt. Express 20, 12793-12798 (2012)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-20-12-12793
Sort: Year | Journal | Reset
References
- B. Huang, S. Jones, B. Brandenburg, and X. Zhuang, “Whole-cell 3D STORM reveals interactions between cellular structures with nanometer-scale resolution,” Nat. Methods5, 1047–1052 (2008). [CrossRef] [PubMed]
- B. Hein, K. Willig, and S. Hell, “Stimulated emission depletion (STED) nanoscopy of a fluorescent protein-labeled organelle inside a living cell,” Proc. Natl. Acad. Sci. USA105, 14271–14276 (2008). [CrossRef] [PubMed]
- E. Rittweger, K. Y. Han, S. E. Irvine, C. Eggeling, and S. W. Hell, “STED microscopy reveals crystal colour centres with nanometric resolution,” Nat. Photonics3, 144–147 (2009). [CrossRef]
- B. Harke, J. Keller, C. K. Ullal, V. Westphal, A. Schönle, and S. W. Hell, “Resolution scaling in STED microscopy,” Opt. Express16, 4154–4162 (2008). [CrossRef] [PubMed]
- M. Leutenegger, C. Eggeling, and S. W. Hell, “Analytical description of STED microscopy performance,” Opt. Express18, 26417–26429 (2010). [CrossRef] [PubMed]
- M. I. Kolobov and C. Fabre, “Quantum limits on optical resolution,” Phys. Rev. Lett.85, 3789–3792 (2000). [CrossRef] [PubMed]
- V. Beskrovnyy and M. Kolobov, “Quantum limits of super-resolution in reconstruction of optical objects,” Phys. Rev. A71, 043802 (2005). [CrossRef]
- Y. Y. Hui, C.-L. Cheng, and H.-C. Chang, “Nanodiamonds for optical bioimaging,” J. Phys. D43, 374021 (2010). [CrossRef]
- D. Wildanger, J. Maze, and S. Hell, “Diffraction unlimited all-optical recording of electron spin resonances,” Phys. Rev. Lett.107, 017601 (2011). [CrossRef] [PubMed]
- A. Caspi, R. Granek, and M. Elbaum, “Enhanced diffusion in active intracellular transport,” Phys. Rev. Lett.85, 5655–5658 (2000). [CrossRef]
- A. Caspi, R. Granek, and M. Elbaum, “Diffusion and directed motion in cellular transport,” Phys. Rev. E66, 011916 (2002). [CrossRef]
- J. T. Verdeyen, Laser Electronics, 3rd ed. (Prentice Hall, New Jersey, 1995).
- M. Weiss, M. Elsner, F. Kartberg, and T. Nilsson, “Anomalous subdiffusion is a measure for cytoplasmic crowding in living cells,” Biophys. J.87, 3518–3524 (2004). [CrossRef] [PubMed]
- L. Nugent-Glandorf and T. T. Perkins, “Measuring 0.1-nm motion in 1 ms in an optical microscope with differential back-focal-plane detection,” Opt. Lett.29, 2611–2613 (2004). [CrossRef] [PubMed]
Cited By |
 Alert me when this paper is cited |
OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.
Related Journal Articles 
- Stochastic transfer function: application to fluorescence microscopy (JOSAA)
- Stochastic transfer function for structured illumination microscopy (JOSAA)
- Wide-field high-resolution solid immersion fluorescence microscopy applying an aplanatic solid immersion lens (AO)
- Wide-field high-resolution structured illumination solid immersion fluorescence microscopy (OL)
- Effect of processing strategies on the stochastic transfer function in structured illumination microscopy (JOSAA)
Related Conference Papers
- Compressive Optical MONTAGE Photography Initiative: Noise and Error Analysis
- Two-Photon Microscopy with Adaptive Illumination Power
- Improved Imaging Property of the Scanning Total Internal Reflection Fluorescence Microscope by the Use of Optimally Polarized Illumination
- Trimodal Optical Microscopy
- Logarithmic Output Active Illumination Microscopy
« Previous Article | Next Article »
OSA is a member of 