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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 20, Iss. 12 — Jun. 4, 2012
  • pp: 13252–13261

3D adaptive optics in a light sheet microscope

Cyril Bourgenot, Christopher D. Saunter, Jonathan M. Taylor, John M. Girkin, and Gordon D. Love  »View Author Affiliations


Optics Express, Vol. 20, Issue 12, pp. 13252-13261 (2012)
http://dx.doi.org/10.1364/OE.20.013252


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Abstract

We report on a single plane illumination microscope (SPIM) incorporating adaptive optics in the imaging arm. We show how aberrations can occur from the sample mounting tube and quantify the aberrations both experimentally and computationally. A wavefront sensorless approach was taken to imaging a green fluorescent protein (GFP) labelled transgenic zebrafish. We show improvements in image quality whilst recording a 3D “z–stack” and show how the aberrations come from varying depths in the fish.

© 2012 OSA

OCIS Codes
(110.0180) Imaging systems : Microscopy
(110.1085) Imaging systems : Adaptive imaging
(110.1080) Imaging systems : Active or adaptive optics

ToC Category:
Adaptive Optics

History
Original Manuscript: March 14, 2012
Revised Manuscript: May 14, 2012
Manuscript Accepted: May 15, 2012
Published: May 29, 2012

Virtual Issues
Vol. 7, Iss. 8 Virtual Journal for Biomedical Optics

Citation
Cyril Bourgenot, Christopher D. Saunter, Jonathan M. Taylor, John M. Girkin, and Gordon D. Love, "3D adaptive optics in a light sheet microscope," Opt. Express 20, 13252-13261 (2012)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-20-12-13252


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