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Polarization-resolved second harmonic generation microscopy with a four-channel Stokes-polarimeterNirmal Mazumder, Jianjun Qiu, Matthew R. Foreman, Carlos Macías Romero, Chih-Wei Hu, Han-Ruei Tsai, Peter Tӧrӧk, and Fu-Jen Kao »View Author Affiliations
Nirmal Mazumder,1
Jianjun Qiu,1
Matthew R. Foreman,2
Carlos Macías Romero,2
Chih-Wei Hu,1
Han-Ruei Tsai,1
Peter Tӧrӧk,2
and Fu-Jen Kao1,*
1Institute of Biophotonics, National Yang-Ming University, 155, Li-Nong St. Taipei 11221, Taiwan 2Blackett Laboratory, Department of Physics, Imperial College London, Prince Consort Road, London SW7 2BZ, UK *Corresponding author: fjkao@ym.edu.tw |
Optics Express, Vol. 20, Issue 13, pp. 14090-14099 (2012)
http://dx.doi.org/10.1364/OE.20.014090
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Abstract
We developed a four-channel photon counting based Stokes-polarimeter for spatial characterization of polarization effects in second harmonic generation (SHG). We have implemented a calibration technique allowing quantitative measurement of polarization parameters, such as the degree of polarization (DOP), degree of linear polarization (DOLP), degree of circular polarization (DOCP), as well as anisotropy from the acquired Stokes parameters. The technique is used as contrast mechanism to characterize the polarization properties from two potassium dihydrogen phosphate (KDP) micro-crystals and collagen type-I in SHG microscopy.
© 2012 OSA
OCIS Codes
(120.0120) Instrumentation, measurement, and metrology : Instrumentation, measurement, and metrology
(120.5410) Instrumentation, measurement, and metrology : Polarimetry
(320.0320) Ultrafast optics : Ultrafast optics
(180.4315) Microscopy : Nonlinear microscopy
ToC Category:
Microscopy
History
Original Manuscript: April 27, 2012
Revised Manuscript: June 1, 2012
Manuscript Accepted: June 1, 2012
Published: June 11, 2012
Virtual Issues
Vol. 7, Iss. 8 Virtual Journal for Biomedical Optics
Citation
Nirmal Mazumder, Jianjun Qiu, Matthew R. Foreman, Carlos Macías Romero, Chih-Wei Hu, Han-Ruei Tsai, Peter Tӧrӧk, and Fu-Jen Kao, "Polarization-resolved second harmonic generation microscopy with a four-channel Stokes-polarimeter," Opt. Express 20, 14090-14099 (2012)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-20-13-14090
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References
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- Y. Sun, W. L. Chen, S. J. Lin, S. H. Jee, Y. F. Chen, L. C. Lin, P. T. C. So, and C. Y. Dong, “Investigating mechanisms of collagen thermal denaturation by high resolution second-harmonic generation imaging,” Biophys. J. 91(7), 2620–2625 (2006). [CrossRef] [PubMed]
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- W. Min, S. Lu, S. Chong, R. Roy, G. R. Holtom, and X. S. Xie, “Imaging chromophores with undetectable fluorescence by stimulated emission microscopy,” Nature 461(7267), 1105–1109 (2009). [CrossRef] [PubMed]
- W. R. Zipfel, R. M. Williams, R. Christie, A. Y. Nikitin, B. T. Hyman, and W. W. Webb, “Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation,” Proc. Natl. Acad. Sci. U.S.A. 100(12), 7075–7080 (2003). [CrossRef] [PubMed]
- S. W. Chu, S. Y. Chen, G. W. Chern, T. H. Tsai, Y. C. Chen, B. L. Lin, and C. K. Sun, “Studies of ?(2)/?(3) tensors in submicron-scaled bio-tissues by polarization harmonics optical microscopy,” Biophys. J. 86(6), 3914–3922 (2004). [CrossRef] [PubMed]
- D. Débarre, W. Supatto, A. M. Pena, A. Fabre, T. Tordjmann, L. Combettes, M. C. Schanne-Klein, and E. Beaurepaire, “Imaging lipid bodies in cells and tissues using third-harmonic generation microscopy,” Nat. Methods 3(1), 47–53 (2006). [CrossRef] [PubMed]
- E. Bélanger, S. Bégin, S. Laffray, Y. De Koninck, R. Vallée, and D. Côté, “Quantitative myelin imaging with coherent anti-Stokes Raman scattering microscopy: alleviating the excitation polarization dependence with circularly polarized laser beams,” Opt. Express 17(21), 18419–18432 (2009). [CrossRef] [PubMed]
- V. Da Costa, R. Wei, R. Lim, C. H. Sun, J. J. Brown, and B. J. F. Wong, “Nondestructive imaging of live human keloid and facial tissue using multiphoton microscopy,” Arch. Facial Plast. Surg. 10(1), 38–43 (2008). [CrossRef] [PubMed]
- E. Bélanger, S. Bégin, S. Laffray, Y. De Koninck, R. Vallée, and D. Côté, “Quantitative myelin imaging with coherent anti-Stokes Raman scattering microscopy: alleviating the excitation polarization dependence with circularly polarized laser beams,” Opt. Express 17(21), 18419–18432 (2009). [CrossRef] [PubMed]
- M. R. Antonelli, A. Pierangelo, T. Novikova, P. Validire, A. Benali, B. Gayet, and A. De Martino, “Mueller matrix imaging of human colon tissue for cancer diagnostics: how Monte Carlo modeling can help in the interpretation of experimental data,” Opt. Express 18(10), 10200–10208 (2010). [CrossRef] [PubMed]
- D. Débarre, W. Supatto, A. M. Pena, A. Fabre, T. Tordjmann, L. Combettes, M. C. Schanne-Klein, and E. Beaurepaire, “Imaging lipid bodies in cells and tissues using third-harmonic generation microscopy,” Nat. Methods 3(1), 47–53 (2006). [CrossRef] [PubMed]
- Y. Sun, W. L. Chen, S. J. Lin, S. H. Jee, Y. F. Chen, L. C. Lin, P. T. C. So, and C. Y. Dong, “Investigating mechanisms of collagen thermal denaturation by high resolution second-harmonic generation imaging,” Biophys. J. 91(7), 2620–2625 (2006). [CrossRef] [PubMed]
- L. M. S. Aas, P. G. Ellingsen, and M. Kildemo, “Near infra-red Mueller matrix imaging system and application to retardance imaging of strain,” Thin Solid Films 519(9), 2737–2741 (2011). [CrossRef]
- D. Débarre, W. Supatto, A. M. Pena, A. Fabre, T. Tordjmann, L. Combettes, M. C. Schanne-Klein, and E. Beaurepaire, “Imaging lipid bodies in cells and tissues using third-harmonic generation microscopy,” Nat. Methods 3(1), 47–53 (2006). [CrossRef] [PubMed]
- S. Brasselet, D. Aït-Belkacem, A. Gasecka, F. Munhoz, S. Brustlein, and S. Brasselet, “Influence of birefringence on polarization resolved nonlinear microscopy and collagen SHG structural imaging,” Opt. Express 18(14), 14859–14870 (2010). [CrossRef] [PubMed]
- P. Schön, F. Munhoz, A. Gasecka, S. Brustlein, and S. Brasselet, “Polarization distortion effects in polarimetric two-photon microscopy,” Opt. Express 16(25), 20891–20901 (2008). [CrossRef] [PubMed]
- M. R. Antonelli, A. Pierangelo, T. Novikova, P. Validire, A. Benali, B. Gayet, and A. De Martino, “Mueller matrix imaging of human colon tissue for cancer diagnostics: how Monte Carlo modeling can help in the interpretation of experimental data,” Opt. Express 18(10), 10200–10208 (2010). [CrossRef] [PubMed]
- N. Ghosh, M. F. G. Wood, and I. A. Vitkin, “Influence of the order of the constituent basis matrices on the Mueller matrix decomposition-derived polarization parameters in complex turbid media such as biological tissue,” Opt. Commun. 283(6), 1200–1208 (2010). [CrossRef]
- E. W. Meijer, E. E. Havinga, and G. L. J. A. Rikken, “Second-harmonic generation in centrosymmetric crystals of chiral molecules,” Phys. Rev. Lett. 65(1), 37–39 (1990). [CrossRef] [PubMed]
- A. Periasamy, D. H. Burns, D. N. Holdren, G. H. Pollack, and K. Trombitás, “A-band shortening in single fibers of frog skeletal muscle,” Biophys. J. 57(4), 815–828 (1990). [CrossRef] [PubMed]
- W. Min, S. Lu, S. Chong, R. Roy, G. R. Holtom, and X. S. Xie, “Imaging chromophores with undetectable fluorescence by stimulated emission microscopy,” Nature 461(7267), 1105–1109 (2009). [CrossRef] [PubMed]
- F. Lu, W. Zheng, and Z. Huang, “Heterodyne polarization coherent anti-Stokes Raman scattering microscopy,” Appl. Phys. Lett. 92(12), 123901 (2008). [CrossRef]
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- Y. Sun, W. L. Chen, S. J. Lin, S. H. Jee, Y. F. Chen, L. C. Lin, P. T. C. So, and C. Y. Dong, “Investigating mechanisms of collagen thermal denaturation by high resolution second-harmonic generation imaging,” Biophys. J. 91(7), 2620–2625 (2006). [CrossRef] [PubMed]
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Nature
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Opt. Commun.
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