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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 20, Iss. 13 — Jun. 18, 2012
  • pp: 14100–14108

Laser oblique scanning optical microscopy (LOSOM) for phase relief imaging

Yichen Ding, Hao Xie, Tong Peng, Yiqing Lu, Dayong Jin, Junlin Teng, Qiushi Ren, and Peng Xi  »View Author Affiliations

Optics Express, Vol. 20, Issue 13, pp. 14100-14108 (2012)

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The visualization of optical phase can provide abundant information when imaging transparent specimen. We present a novel phase sensitive imaging design capable of obtaining phase contours of transparent biological cells through laser oblique scanning optical microscope (LOSOM). LOSOM is based on the introduction of a fluorescent medium behind the specimen to generate a differential phase-sensitive image, thus, the complicated phase retardation alignment procedure associated with differential interference contrast (DIC) microscopy can be eliminated. Moreover, multi-modality fluorescence and phase relief imaging can be attained in a single system with fluorescently labeled specimens.

© 2012 OSA

OCIS Codes
(120.5060) Instrumentation, measurement, and metrology : Phase modulation
(170.0180) Medical optics and biotechnology : Microscopy
(180.5810) Microscopy : Scanning microscopy

ToC Category:

Original Manuscript: May 7, 2012
Revised Manuscript: May 28, 2012
Manuscript Accepted: May 31, 2012
Published: June 11, 2012

Virtual Issues
Vol. 7, Iss. 8 Virtual Journal for Biomedical Optics

Yichen Ding, Hao Xie, Tong Peng, Yiqing Lu, Dayong Jin, Junlin Teng, Qiushi Ren, and Peng Xi, "Laser oblique scanning optical microscopy (LOSOM) for phase relief imaging," Opt. Express 20, 14100-14108 (2012)

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