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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 20, Iss. 14 — Jul. 2, 2012
  • pp: 15377–15384

SLM-based off-axis Fourier filtering in microscopy with white light illumination

Ruth Steiger, Stefan Bernet, and Monika Ritsch-Marte  »View Author Affiliations

Optics Express, Vol. 20, Issue 14, pp. 15377-15384 (2012)

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In various microscopy applications spatial light modulators (SLMs) are used as programmable Fourier filters to realize different optical contrast enhancement methods. It is often advantageous to use the SLM in off-axis configuration, where the filtered image wave is sent into the first diffraction order of a blazed grating superposed to the phase mask on the SLM. Because of dispersion this approach is, however, typically limited to spectrally narrowband illumination. Here we suggest a method involving a grating for pre-compensation, which allows one to use spectrally broadband (even thermal) light in SLM-based Fourier filtering. The proposed approach is demonstrated by multicolor imaging of amplitude and phase objects, such as a resolution target, onion epidermal cells and human epithelial cheek cells.

© 2012 OSA

OCIS Codes
(070.6110) Fourier optics and signal processing : Spatial filtering
(170.0180) Medical optics and biotechnology : Microscopy
(230.1950) Optical devices : Diffraction gratings
(230.3720) Optical devices : Liquid-crystal devices
(260.2030) Physical optics : Dispersion

ToC Category:

Original Manuscript: April 24, 2012
Revised Manuscript: June 15, 2012
Manuscript Accepted: June 20, 2012
Published: June 25, 2012

Virtual Issues
Vol. 7, Iss. 9 Virtual Journal for Biomedical Optics

Ruth Steiger, Stefan Bernet, and Monika Ritsch-Marte, "SLM-based off-axis Fourier filtering in microscopy with white light illumination," Opt. Express 20, 15377-15384 (2012)

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