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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 20, Iss. 17 — Aug. 13, 2012
  • pp: 19232–19254

Hard X-ray imaging of bacterial cells: nano-diffraction and ptychographic reconstruction

R. N. Wilke, M. Priebe, M. Bartels, K. Giewekemeyer, A. Diaz, P. Karvinen, and T. Salditt  »View Author Affiliations

Optics Express, Vol. 20, Issue 17, pp. 19232-19254 (2012)

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Ptychographic coherent X-ray diffractive imaging (PCDI) has been combined with nano-focus X-ray diffraction to study the structure and density distribution of unstained and unsliced bacterial cells, using a hard X-ray beam of 6.2keV photon energy, focused to about 90nm by a Fresnel zone plate lens. While PCDI provides images of the bacteria with quantitative contrast in real space with a resolution well below the beam size at the sample, spatially resolved small angle X-ray scattering using the same Fresnel zone plate (cellular nano-diffraction) provides structural information at highest resolution in reciprocal space up to 2nm−1. We show how the real and reciprocal space approach can be used synergistically on the same sample and with the same setup. In addition, we present 3D hard X-ray imaging of unstained bacterial cells by a combination of ptychography and tomography.

© 2012 OSA

OCIS Codes
(100.5070) Image processing : Phase retrieval
(100.6950) Image processing : Tomographic image processing
(170.1530) Medical optics and biotechnology : Cell analysis
(340.7440) X-ray optics : X-ray imaging

ToC Category:
Image Processing

Original Manuscript: May 10, 2012
Revised Manuscript: June 8, 2012
Manuscript Accepted: June 13, 2012
Published: August 8, 2012

Virtual Issues
Vol. 7, Iss. 10 Virtual Journal for Biomedical Optics

R. N. Wilke, M. Priebe, M. Bartels, K. Giewekemeyer, A. Diaz, P. Karvinen, and T. Salditt, "Hard X-ray imaging of bacterial cells: nano-diffraction and ptychographic reconstruction," Opt. Express 20, 19232-19254 (2012)

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