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Reconstruction of objects above and below the objective focal plane with dimensional fidelity by FINCH fluorescence microscopy |
Optics Express, Vol. 20, Issue 18, pp. 19822-19835 (2012)
http://dx.doi.org/10.1364/OE.20.019822
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Abstract
Fresnel Incoherent Correlation Holography (FINCH) can faithfully reproduce objects above and below the optical plane of focus. However, as in optical imaging, the transverse magnification and optimal reconstruction depth changes based on the longitudinal distance of objects from the focal plane of the input lens with the exception that objects above and below the focal plane are in focus with FINCH and out of focus by standard optical imaging. We have analyzed these effects both theoretically and experimentally for two configurations of a FINCH fluorescence microscopy system. This information has been used to reconstruct a test planar object placed above or below the optical plane of focus with high dimensional and image fidelity. Because FINCH is inherently a super-resolving system, this advance makes it possible to create super-resolved 3D images from FINCH holograms.
© 2012 OSA
OCIS Codes
(090.1760) Holography : Computer holography
(090.1970) Holography : Diffractive optics
(090.2880) Holography : Holographic interferometry
(100.6890) Image processing : Three-dimensional image processing
(110.0180) Imaging systems : Microscopy
(110.6880) Imaging systems : Three-dimensional image acquisition
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(260.2510) Physical optics : Fluorescence
(090.1995) Holography : Digital holography
ToC Category:
Holography
History
Original Manuscript: July 9, 2012
Revised Manuscript: August 2, 2012
Manuscript Accepted: August 3, 2012
Published: August 14, 2012
Virtual Issues
Vol. 7, Iss. 10 Virtual Journal for Biomedical Optics
Citation
Nisan Siegel, Joseph Rosen, and Gary Brooker, "Reconstruction of objects above and below the objective focal plane with dimensional fidelity by FINCH fluorescence microscopy," Opt. Express 20, 19822-19835 (2012)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-20-18-19822
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References
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