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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 20, Iss. 19 — Sep. 10, 2012
  • pp: 21010–21018

Alignment-free, all-spliced fiber laser source for CARS microscopy based on four-wave-mixing

Martin Baumgartl, Thomas Gottschall, Javier Abreu-Afonso, Antonio Díez, Tobias Meyer, Benjamin Dietzek, Manfred Rothhardt, Jürgen Popp, Jens Limpert, and Andreas Tünnermann  »View Author Affiliations

Optics Express, Vol. 20, Issue 19, pp. 21010-21018 (2012)

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An environmentally-stable low-repetition rate fiber oscillator is developed to produce narrow-bandwidth pulses with several tens of picoseconds duration. Based on this oscillator an alignment-free all-fiber laser for multi-photon microscopy is realized using in-fiber frequency conversion based on four-wave-mixing. Both pump and Stokes pulses for coherent anti-Stokes Raman scattering (CARS) microscopy are readily available from one fiber end, intrinsically overlapped in space and time, which drastically simplifies the experimental handling for the user. The complete laser setup is mounted on a home-built laser scanning microscope with small footprint. High-quality multimodal microscope images of biological tissue are presented probing the CH-stretching resonance of lipids at an anti-Stokes Raman-shift of 2845 cm−1 and second-harmonic generation of collagen. Due to its simplicity, compactness, maintenance-free operation, and ease-of-use the presented low-cost laser is an ideal source for bio-medical applications outside laser laboratories and in particular inside clinics.

© 2012 OSA

OCIS Codes
(060.2320) Fiber optics and optical communications : Fiber optics amplifiers and oscillators
(140.4050) Lasers and laser optics : Mode-locked lasers
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(190.4380) Nonlinear optics : Nonlinear optics, four-wave mixing
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:

Original Manuscript: July 3, 2012
Revised Manuscript: August 3, 2012
Manuscript Accepted: August 5, 2012
Published: August 29, 2012

Virtual Issues
Vol. 7, Iss. 11 Virtual Journal for Biomedical Optics

Martin Baumgartl, Thomas Gottschall, Javier Abreu-Afonso, Antonio Díez, Tobias Meyer, Benjamin Dietzek, Manfred Rothhardt, Jürgen Popp, Jens Limpert, and Andreas Tünnermann, "Alignment-free, all-spliced fiber laser source for CARS microscopy based on four-wave-mixing," Opt. Express 20, 21010-21018 (2012)

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