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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 20, Iss. 2 — Jan. 16, 2012
  • pp: 816–826

Assessing the contribution of cell body and intracellular organelles to the backward light scattering

Maxim Kalashnikov, Wonshik Choi, Martin Hunter, Chung-Chieh Yu, Ramachandra R. Dasari, and Michael S. Feld  »View Author Affiliations

Optics Express, Vol. 20, Issue 2, pp. 816-826 (2012)

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We report a method of assessing the contribution of whole cell body and its nucleus to the clinically most relevant backward light scattering. We first construct an experimental system that can measure forward scattering and use the system to precisely extract the optical properties of a specimen such as the refractive index contrast, size distribution, and their density. A system that can simultaneously detect the backscattered light is installed to collect the backscattering for the same specimen. By comparing the measured backscattering spectrum with that estimated from the parameters determined by the forward scattering experiment, the contribution of cell body and nucleus to the backward light scattering is quantitatively assessed. For the HeLa cells in suspension, we found that the cell body contributes less than 10% and cell nucleus on the order of 0.1% to the total backscattering signal. Quantitative determination of the origin of backscattered light may help design a system that aims for detecting particular structure of biological tissues.

© 2012 OSA

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(290.1350) Scattering : Backscattering
(290.4020) Scattering : Mie theory
(290.5820) Scattering : Scattering measurements
(290.2558) Scattering : Forward scattering

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: August 31, 2011
Revised Manuscript: October 28, 2011
Manuscript Accepted: December 10, 2011
Published: January 3, 2012

Virtual Issues
Vol. 7, Iss. 3 Virtual Journal for Biomedical Optics

Maxim Kalashnikov, Wonshik Choi, Martin Hunter, Chung-Chieh Yu, Ramachandra R. Dasari, and Michael S. Feld, "Assessing the contribution of cell body and intracellular organelles to the backward light scattering," Opt. Express 20, 816-826 (2012)

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