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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 20, Iss. 2 — Jan. 16, 2012
  • pp: 960–965

Optical refocusing three-dimensional wide-field fluorescence lifetime imaging microscopy

Qiang Wu, Shangyu Guo, Yinxing Ma, Feng Gao, Chengliang Yang, Ming Yang, Xuanyi Yu, Xinzheng Zhang, Romano A. Rupp, and Jingjun Xu  »View Author Affiliations


Optics Express, Vol. 20, Issue 2, pp. 960-965 (2012)
http://dx.doi.org/10.1364/OE.20.000960


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Abstract

Three-dimensional fluorescence lifetime microscopy is achieved by combining wide-field fluorescence lifetime imaging with a remote optical refocusing method. As required for some applications in dynamic research for physics, chemistry, or biology, it is thereby not necessary to move the sample, i.e., the specimen is not disturbed during measurement. Using a fluorescent microsphere the performance of the system has been tested successfully with respect to three-dimensional fluorescence lifetime microscopy as well as time-resolved fluorescence spectroscopy.

© 2012 OSA

OCIS Codes
(170.6920) Medical optics and biotechnology : Time-resolved imaging
(300.6500) Spectroscopy : Spectroscopy, time-resolved
(320.7150) Ultrafast optics : Ultrafast spectroscopy
(100.0118) Image processing : Imaging ultrafast phenomena

ToC Category:
Microscopy

History
Original Manuscript: November 1, 2011
Manuscript Accepted: December 6, 2011
Published: January 4, 2012

Virtual Issues
Vol. 7, Iss. 3 Virtual Journal for Biomedical Optics

Citation
Qiang Wu, Shangyu Guo, Yinxing Ma, Feng Gao, Chengliang Yang, Ming Yang, Xuanyi Yu, Xinzheng Zhang, Romano A. Rupp, and Jingjun Xu, "Optical refocusing three-dimensional wide-field fluorescence lifetime imaging microscopy," Opt. Express 20, 960-965 (2012)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-20-2-960


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References

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