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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 20, Iss. 20 — Sep. 24, 2012
  • pp: 22245–22251

A novel method of diffraction imaging flow cytometry for sizing microspheres

Sa Yu, Jun Zhang, Marina S. Moran, Jun Q. Lu, Yuanming Feng, and Xin-Hua Hu  »View Author Affiliations


Optics Express, Vol. 20, Issue 20, pp. 22245-22251 (2012)
http://dx.doi.org/10.1364/OE.20.022245


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Abstract

We report a novel method of diffraction imaging flow cytometry to measure and analyze size distribution of microspheres. An automated and robust image processing software based on the short-time-Fourier-transform algorithm has been developed to analyze the characteristic and spatially varying oscillations of side scatters recorded as a diffraction image. Our results demonstrate that the new method allows accurate and rapid determination of single microspheres’ diameters ranging from 1 to 100μm. The capacity for analysis of light scattering by two-sphere aggregates has been demonstrated but analytical tools for characterization of aggregates by multiple microspheres remain to be developed.

© 2012 OSA

OCIS Codes
(110.1650) Imaging systems : Coherence imaging
(290.5850) Scattering : Scattering, particles

ToC Category:
Imaging Systems

History
Original Manuscript: June 29, 2012
Revised Manuscript: August 23, 2012
Manuscript Accepted: August 24, 2012
Published: September 13, 2012

Citation
Sa Yu, Jun Zhang, Marina S. Moran, Jun Q. Lu, Yuanming Feng, and Xin-Hua Hu, "A novel method of diffraction imaging flow cytometry for sizing microspheres," Opt. Express 20, 22245-22251 (2012)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-20-20-22245


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