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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 20, Iss. 23 — Nov. 5, 2012
  • pp: 25979–25989

Laser divided-aperture differential confocal sensing technology with improved axial resolution

Weiqian Zhao, Chao Liu, and Lirong Qiu  »View Author Affiliations

Optics Express, Vol. 20, Issue 23, pp. 25979-25989 (2012)

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In this study, we found that the axial response curve of divided-aperture confocal microscopy has a shift while the point detector has a transverse offset from the optical axis. Based on this, a novel laser divided-aperture differential confocal sensing technology (LDDCST) with absolute zero and high axial resolution, as well as an LDDCST-based sensor, is proposed. LDDCST sets two micro-regions as virtual pinholes that are symmetrical to the optical axis along the xd direction on the focal plane of the divided-aperture confocal system to achieve the spot-division detection and to simplify the detection system, uses differential subtraction of two intensity responses simultaneously detected from the two micro-regions to achieve high axial resolution absolute measurement and low noise, and considers both resolution and measurement range by adjusting virtual pinholes in software. Theoretical analyses and packaged LDDCST sensor experiments indicate that LDDCST has high axial resolution as well as strong anti-interference and sectioning detection capability.

© 2012 OSA

OCIS Codes
(120.0120) Instrumentation, measurement, and metrology : Instrumentation, measurement, and metrology
(120.6650) Instrumentation, measurement, and metrology : Surface measurements, figure
(120.6660) Instrumentation, measurement, and metrology : Surface measurements, roughness
(130.6010) Integrated optics : Sensors
(180.1790) Microscopy : Confocal microscopy

ToC Category:

Original Manuscript: September 7, 2012
Revised Manuscript: October 21, 2012
Manuscript Accepted: October 23, 2012
Published: November 2, 2012

Virtual Issues
Vol. 7, Iss. 12 Virtual Journal for Biomedical Optics

Weiqian Zhao, Chao Liu, and Lirong Qiu, "Laser divided-aperture differential confocal sensing technology with improved axial resolution," Opt. Express 20, 25979-25989 (2012)

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  1. Z. Li, K. Herrmann, and F. Pohlenz, “Lateral scanning confocal microscopy for the determination of in-plane displacements of microelectromechanical systems devices,” Opt. Lett.32(12), 1743–1745 (2007). [CrossRef] [PubMed]
  2. J. F. Aguilar, M. Lera, and C. J. R. Sheppard, “Imaging of spheres and surface profiling by confocal microscopy,” Appl. Opt.39(25), 4621–4628 (2000). [CrossRef] [PubMed]
  3. H. Yu, T. Chen, and J. Qu, “Improving FRET efficiency measurement in confocal microscopy imaging,” Chin. Opt. Lett.8(10), 947–949 (2010). [CrossRef]
  4. C. L. Arrasmith, D. L. Dickensheets, and A. Mahadevan-Jansen, “MEMS-based handheld confocal microscope for in-vivo skin imaging,” Opt. Express18(4), 3805–3819 (2010). [CrossRef] [PubMed]
  5. C. J. Koester, “Scanning mirror microscope with optical sectioning characteristics: applications in ophthalmology,” Appl. Opt.19(11), 1749–1757 (1980). [CrossRef] [PubMed]
  6. C. J. Koester, S. M. Khanna, H. D. Rosskothen, R. B. Tackaberry, and M. Ulfendahl, “Confocal slit divided-aperture microscope: applications in ear research,” Appl. Opt.33(4), 702–708 (1994). [CrossRef] [PubMed]
  7. P. J. Dwyer, C. A. DiMarzio, J. M. Zavislan, W. J. Fox, and M. Rajadhyaksha, “Confocal reflectance theta line scanning microscope for imaging human skin in vivo,” Opt. Lett.31(7), 942–944 (2006). [CrossRef] [PubMed]
  8. P. J. Dwyer, C. A. DiMarzio, and M. Rajadhyaksha, “Confocal theta line-scanning microscope for imaging human tissues,” Appl. Opt.46(10), 1843–1851 (2007). [CrossRef] [PubMed]
  9. C. J. R. Sheppard, W. Gong, and K. Si, “The divided aperture technique for microscopy through scattering media,” Opt. Express16(21), 17031–17038 (2008). [CrossRef] [PubMed]
  10. K. Si, W. Gong, and C. J. R. Sheppard, “Three-dimensional coherent transfer function for a confocal microscope with two D-shaped pupils,” Appl. Opt.48(5), 810–817 (2009). [CrossRef] [PubMed]
  11. W. Gong, K. Si, and C. J. R. Sheppard, “Optimization of axial resolution in a confocal microscope with D-shaped apertures,” Appl. Opt.48(20), 3998–4002 (2009). [CrossRef] [PubMed]
  12. W. Gong, K. Si, and C. J. R. Sheppard, “Improvements in confocal microscopy imaging using serrated divided apertures,” Opt. Commun.282(19), 3846–3849 (2009). [CrossRef]
  13. W. Zhao, J. Tan, and L. Qiu, “Bipolar absolute differential confocal approach to higher spatial resolution,” Opt. Express12(21), 5013–5021 (2004). [CrossRef] [PubMed]
  14. M. Gu, Principles of Three-Dimensional Imaging in Confocal Microscopes (World Scientific Publishing, 1996), chap. 3.

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