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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 20, Iss. 24 — Nov. 19, 2012
  • pp: 26681–26695

Super-resolution photon-efficient imaging by nanometric double-helix point spread function localization of emitters (SPINDLE)

Ginni Grover, Keith DeLuca, Sean Quirin, Jennifer DeLuca, and Rafael Piestun  »View Author Affiliations

Optics Express, Vol. 20, Issue 24, pp. 26681-26695 (2012)

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Super-resolution imaging with photo-activatable or photo-switchable probes is a promising tool in biological applications to reveal previously unresolved intra-cellular details with visible light. This field benefits from developments in the areas of molecular probes, optical systems, and computational post-processing of the data. The joint design of optics and reconstruction processes using double-helix point spread functions (DH-PSF) provides high resolution three-dimensional (3D) imaging over a long depth-of-field. We demonstrate for the first time a method integrating a Fisher information efficient DH-PSF design, a surface relief optical phase mask, and an optimal 3D localization estimator. 3D super-resolution imaging using photo-switchable dyes reveals the 3D microtubule network in mammalian cells with localization precision approaching the information theoretical limit over a depth of 1.2 µm.

© 2012 OSA

OCIS Codes
(110.4850) Imaging systems : Optical transfer functions
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(110.1758) Imaging systems : Computational imaging
(050.4865) Diffraction and gratings : Optical vortices

ToC Category:
Imaging Systems

Original Manuscript: August 6, 2012
Revised Manuscript: October 16, 2012
Manuscript Accepted: November 5, 2012
Published: November 12, 2012

Virtual Issues
Vol. 7, Iss. 12 Virtual Journal for Biomedical Optics

Ginni Grover, Keith DeLuca, Sean Quirin, Jennifer DeLuca, and Rafael Piestun, "Super-resolution photon-efficient imaging by nanometric double-helix point spread function localization of emitters (SPINDLE)," Opt. Express 20, 26681-26695 (2012)

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